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| Product Name | High-Accuracy RT SuperMix for qPCR with gDNA Removal |
| Catalog No. | NATR-HMM-0170 |
| Description | A qPCR-focused reverse-transcription premix combining a high-performance reverse transcriptase, an optimized buffer system and genomic DNA removal reagents for consistent cDNA preparation. |
| Intended Use | First-strand cDNA synthesis; reverse transcription; gene expression analysis; two-step RT-qPCR. |
| Principle / Technology | An optimized reverse-transcription system converts RNA into cDNA after removal of genomic DNA contamination. |
| Sample Type | Total RNA. |
| Performance Range / Specifications | 100 reactions; qPCR amplification efficiency close to 100% across the tested RNA input range, with stable replicate data and low well-to-well CV. |
| Specificity | Optimized for reproducible qPCR-oriented cDNA synthesis across different RNA copy numbers. |
| Reaction Conditions / Protocol | Perform genomic DNA removal followed by reverse transcription according to the supplied workflow. |
| Components / Formulation | High-performance reverse transcriptase, optimized RT buffer system and genomic DNA removal module. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions. |
| Product Form | qPCR reverse-transcription supermix with genomic DNA removal module. |
| Quality Control | Evaluated by gradient-template reverse transcription and subsequent qPCR efficiency and repeatability analysis. |
| Key Features | High amplification accuracy; stable reverse-transcription performance; low replicate CV; convenient premixed format. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Resulting cDNA is compatible with dye-based and probe-based qPCR. |
| Application Notes / Precautions | Mix gently and keep RNA and reagents free of nuclease contamination. |
For research use only, not for clinical use.
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