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| Product Name | Glutamine/Glutamate Colorimetric Dual Detection Kit |
| Catalog No. | CMTR-HMM-0068 |
| Description | A two-step colorimetric assay kit enabling sequential measurement of glutamine and glutamate concentrations from the same sample aliquot. The first step detects endogenous glutamate via glutamate dehydrogenase-catalyzed conversion producing NADH. In the second step, glutaminase converts glutamine to additional glutamate, which is then quantified by the same detection system. Subtraction of the initial glutamate reading from the total yields glutamine concentration. |
| Intended Use | Sequential dual measurement of glutamine and glutamate concentrations in cell culture media, cell lysates, and biological fluids. |
| Principle / Technology | Enzymatic cycling of glutamate dehydrogenase and glutaminase with NADH colorimetric detection |
| Detection Method | Colorimetric (absorbance at 450 nm) |
| Sample Type | Cell culture medium, cell and tissue lysates, serum, plasma |
| Performance Range / Specifications | Linear range: glutamine 0.02–2 mM; glutamate 0.01–1 mM |
| Sensitivity / LOD | Detection limit: glutamine 10 µM; glutamate 5 µM |
| Specificity | Glutamate dehydrogenase specific to L-glutamate; glutaminase specific to L-glutamine; D-isomers not detected |
| Reaction Conditions / Protocol | Step 1: add glutamate detection reagent, incubate 30 min at 37 °C, read absorbance; Step 2: add glutaminase conversion reagent, incubate 30 min, read absorbance; calculate glutamine by subtraction |
| Components / Formulation | Glutamate detection reagent (glutamate dehydrogenase, NAD+, WST-1), glutaminase conversion reagent, glutamate standard, glutamine standard, assay buffer |
| Storage Conditions | –20 °C for enzymes and standards; 2–8 °C for buffer |
| Shelf Life | 6 months at –20 °C |
| Package Specifications | 100 tests |
| Product Form | Liquid reagents; lyophilized standards |
| Quality Control | Standards calibrated by quantitative NMR; spike-recovery 95–105% in cell culture medium matrix |
| Key Features | Sequential measurement from single sample aliquot eliminates well-to-well variation in dual-analyte comparison |
| Purity | Reagent grade; fluorescent probes ≥95% pure by HPLC |
| Concentration | As specified per kit format; probe stock solutions in DMSO as specified |
| Activity / Unit Definition | Standard curve linearity R² ≥0.99 per lot; dynamic range as specified |
| Molecular Weight | Varies by probe and buffer component |
| Source / Origin | Synthetic fluorescent probes; recombinant enzymes; purified metabolite standards |
| pH Range / Optimal pH | pH 7.2–7.4 for cell-based assays; pH varies for biochemical metabolite detection |
| Shipping Conditions | Cold packs or dry ice for probes and enzymes; protect from light |
| Expiration Date / Stability | 6–12 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 |
| Compatibility | Compatible with fluorescence microplate readers, luminometers, and spectrophotometers |
| Recommended Buffer System | Assay-specific buffer optimized per product protocol |
| Application Notes / Precautions | Normalize signal to cell number or protein content; avoid probe photobleaching; include appropriate antioxidant controls for ROS assays |
| Batch-to-Batch Consistency | Standard curve parameters within ±15% of reference lot |
For research use only, not for clinical use.
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