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| Product Name | Gel DNA Recovery and Cleanup Mini Kit |
| Catalog No. | DREK-0098 |
| Description | A dual-purpose silica-column kit for recovering DNA from TAE or TBE agarose gels and purifying PCR products or other crude DNA preparations. The workflow requires no column equilibration. |
| Intended Use | Agarose gel DNA recovery and cleanup of PCR or enzymatic reaction products before ligation, transformation, restriction digestion, in vitro transcription, sequencing or microinjection. |
| Principle / Technology | Gel dissolution or direct sample conditioning followed by selective silica-membrane binding, washing and elution. |
| Detection Method | Downstream DNA analysis. |
| Sample Type | DNA bands in TAE or TBE agarose gels, PCR products, enzymatic reaction mixtures and other crude DNA preparations. |
| Performance Range / Specifications | DNA fragment range: 70 bp-20 kb; recovery efficiency up to 80%; approximately 10-15 minutes; 100 reactions. |
| Specificity | Silica-membrane purification removes agarose, proteins, organic compounds, salts and oligonucleotide primers while recovering target DNA. |
| Reaction Conditions / Protocol | Dissolve the gel slice with an equal volume of solubilization reagent or condition the crude DNA sample, bind to the spin column, wash and elute. |
| Components / Formulation | Gel-solubilization and binding reagent, wash and elution buffers, silica spin columns and collection tubes. |
| Storage Conditions | Store at 15-25°C. |
| Package Specifications | 100 reactions. |
| Product Form | Gel extraction and DNA cleanup spin-column kit. |
| Quality Control | Fragment recovery, purity and downstream ligation or amplification performance are evaluated across the stated size range. |
| Key Features | 70 bp-20 kb recovery range; up to 80% recovery; no column equilibration; approximately 10-15-minute workflow; gel extraction and reaction cleanup in one kit. |
| Shipping Conditions | Ship at room temperature. |
| Compatibility | Ligation, transformation, restriction digestion, in vitro transcription, PCR, sequencing and microinjection workflows. |
| Application Notes / Precautions | Minimize UV exposure during gel excision and avoid excessive gel mass relative to the dissolution reagent. |
| Batch-to-Batch Consistency | DNA recovery and purity are controlled across production batches. |
For research use only, not for clinical use.
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