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| Product Name | Four-Plex One-Step RT-qPCR Master Mix with 0.1 pg Sensitivity |
| Catalog No. | NATR-HMM-0249 |
| Description | Alta DiagnoTech one-step multiplex RT-qPCR master mix combines thermostable reverse transcriptase, hot-start Taq and RNase inhibitor for probe-based RNA amplification. |
| Intended Use | For one-step multiplex fluorescent quantitative PCR using RNA templates. |
| Principle / Technology | Reverse transcription and qPCR occur in one tube. Thermostable reverse transcriptase synthesizes first-strand cDNA, hot-start Taq performs amplification and UDG reduces carryover contamination. |
| Detection Method | TaqMan probe-based real-time fluorescence detection |
| Performance Range / Specifications | Detection sensitivity reaches 0.1 pg and the formulation supports up to four targets in one reaction. |
| Components / Formulation | Thermostable reverse transcriptase, antibody-modified hot-start Taq DNA polymerase, RNase inhibitor, UDG and a buffer containing multiplex-efficiency enhancers and nonspecific-amplification suppressors. |
| Storage Conditions | Store at -25°C to -15°C. |
| Package Specifications | Available in 100-, 1,000- and 10,000-reaction formats. |
| Product Form | One-step liquid RT-qPCR master mix |
| Shipping Conditions | Ship at 0°C or below. |
| Product Type | Four-plex one-step probe RT-qPCR master mix |
| Function / Efficacy | Provides stable, sensitive multiplex RNA amplification with carryover contamination control. |
For research use only, not for clinical use.
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