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| Product Name | Flow Cytometer Sheath Fluid and Fluidics Cleaning Solution |
| Catalog No. | ATRCS-0023 |
| Description | A dual-purpose sheath fluid and fluidics line cleaning solution for flow cytometers. When used as directed, it serves as an isotonic sheath fluid for routine sample analysis while its surfactant system actively prevents salt crystal buildup, dye adsorption, and protein fouling in the flow cell, sample injection tube, and fluidic tubing. Also suitable for deep cleaning cycles between experiments. |
| Intended Use | Continuous use as sheath fluid and periodic deep cleaning of flow cytometer fluidic systems including BD FACSCanto, FACSCalibur, LSRFortessa, Beckman Coulter CytoFLEX, and other commercial platforms. |
| Principle / Technology | Isotonic electrolyte-balanced buffer with anti-fouling surfactant system that prevents particulate and protein adhesion to quartz flow cell surfaces |
| Detection Method | For sheath fluid: use undiluted in sheath tank. For deep cleaning: circulate undiluted for 15–30 minutes, followed by deionized water flush |
| Components / Formulation | Sodium chloride, potassium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate, non-ionic surfactant (Pluronic F-68), sodium azide (<0.1%), deionized water |
| Storage Conditions | Store at 15–30 °C; protect from direct sunlight; keep container tightly closed |
| Shelf Life | 24 months from manufacture date; use within 90 days after opening |
| Package Specifications | 5 L, 10 L, 20 L HDPE cubitainers |
| Product Form | Clear colorless liquid, ready-to-use |
| Key Features | Dual-purpose formulation reduces need for separate sheath fluid and cleaning solution; anti-fouling surfactants maintain consistent flow cell performance; isotonic formulation prevents cell swelling or shrinkage; 0.22 µm filtered and tested particle-free; validated for consistent CV <2% on alignment beads |
| Purity | All salts meet ACS reagent specifications; endotoxin <0.05 EU/mL |
| Concentration | As specified (concentrate or ready-to-use) |
| Activity / Unit Definition | Cleaning efficacy validated by instrument carryover testing |
| Molecular Weight | Not applicable — detergent/surfactant formulations |
| Source / Origin | Synthetic detergent and surfactant components |
| pH Range / Optimal pH | pH 7.35–7.45 (at 25 °C) |
| Shipping Conditions | Ambient temperature; no special handling required |
| Expiration Date / Stability | 24 months under recommended storage; 90 days after opening; discard if turbidity or microbial growth is observed |
| Regulatory / Compliance | For laboratory use; not classified as dangerous goods; manufactured under ISO 9001; each lot certified endotoxin-free |
| Compatibility | Compatible with all flow cytometer fluidic materials; validated on BD, Beckman Coulter, Thermo Fisher Attune, Sony, and Miltenyi flow cytometer platforms; not for use with mass cytometry (CyTOF) systems |
| Recommended Buffer System | Use undiluted or dilute per instrument manufacturer specification |
| Application Notes / Precautions | For daily use as sheath fluid, top up sheath tank as needed. For cleaning cycle, run undiluted solution for 15–30 minutes at standard sheath pressure, then flush with 2 L deionized water before resuming sample analysis. For heavily fouled flow cells, an extended 60-minute clean may be necessary. |
| Batch-to-Batch Consistency | pH and conductivity within ±5% of reference lot; cleaning efficacy validated |
For research use only, not for clinical use.
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