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| Product Name | First-Strand cDNA Synthesis Kit with gDNA Removal |
| Catalog No. | NATR-HMM-0178 |
| Description | A general-purpose first-strand cDNA synthesis kit with a rapid genomic DNA removal module and supplied oligo(dT)23VN and random hexamer primers. |
| Intended Use | First-strand cDNA synthesis; reverse transcription; PCR; qPCR. |
| Principle / Technology | Genomic DNA is removed before reverse transcriptase synthesizes cDNA using oligo(dT)23VN, random hexamers or application-specific primer combinations. |
| Sample Type | Total RNA. |
| Performance Range / Specifications | 50 or 100 reactions at 20 µL per reaction; total RNA input from 10 pg to 5 µg; amplifiable cDNA fragments above 7.1 kb and full-length synthesis capability up to 20 kb; genomic DNA removal in approximately 2 minutes. |
| Specificity | Rapid genomic DNA removal reduces background from contaminating DNA; supplied primers provide broad transcript coverage. |
| Reaction Conditions / Protocol | Treat RNA with the genomic DNA removal mixture for approximately 2 minutes, then synthesize first-strand cDNA using the selected primer system. |
| Components / Formulation | Reverse-transcription enzyme system, genomic DNA removal module, oligo(dT)23VN primers and random hexamer primers. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 50 reactions (20 µL per reaction); 100 reactions (20 µL per reaction). |
| Product Form | First-strand cDNA synthesis kit with genomic DNA removal. |
| Quality Control | Genomic DNA removal and long-target cDNA synthesis are evaluated using subsequent amplification assays. |
| Key Features | Broad RNA input range; rapid genomic DNA removal; long-cDNA capability; high-temperature and impurity tolerance; flexible primer options. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with downstream PCR and qPCR. |
| Application Notes / Precautions | Select oligo(dT)23VN, random hexamers or gene-specific primers according to the RNA template and downstream target. |
For research use only, not for clinical use.
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