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| Product Name | First-Strand cDNA Synthesis Kit |
| Catalog No. | NATR-HMM-0179 |
| Description | A general-purpose reverse-transcription kit for high-efficiency first-strand cDNA synthesis from total RNA or poly(A)+ RNA using flexible primer options. |
| Intended Use | First-strand cDNA synthesis; reverse transcription; second-strand cDNA synthesis; hybridization; PCR. |
| Principle / Technology | Reverse transcriptase converts RNA to first-strand cDNA using oligo(dT)23VN, random hexamers or gene-specific primers. |
| Sample Type | Total RNA or poly(A)+ mRNA. |
| Performance Range / Specifications | 50 or 100 reactions at 20 µL per reaction; RNA input from 1 pg to 5 µg; amplifiable cDNA fragments above 15 kb and full-length synthesis capability up to 20 kb. |
| Specificity | Flexible priming supports polyadenylated transcripts, broad RNA coverage or gene-specific reverse transcription. |
| Reaction Conditions / Protocol | Combine RNA with the selected primer and reverse-transcription reagents, then run the recommended first-strand cDNA synthesis program. |
| Components / Formulation | Reverse-transcription enzyme system, optimized reaction buffer, oligo(dT)23VN primers and random hexamer primers. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 50 reactions (20 µL per reaction); 100 reactions (20 µL per reaction). |
| Product Form | First-strand cDNA synthesis kit. |
| Quality Control | Evaluated by long-target cDNA synthesis and downstream amplification. |
| Key Features | Very broad RNA input range; long full-length cDNA synthesis; flexible primer selection; suitable for multiple downstream molecular biology applications. |
| Compatibility | Compatible with downstream PCR, second-strand cDNA synthesis and hybridization workflows. |
| Application Notes / Precautions | This formulation does not include a genomic DNA removal step. Use oligo(dT)23VN, random hexamers or gene-specific primers according to the application. |
For research use only, not for clinical use.
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