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| Product Name | Enhanced Endotoxin-Free Plasmid Maxi Extraction Kit |
| Catalog No. | DREK-0087 |
| Description | A large-scale plasmid purification kit combining alkaline lysis, color-indicated neutralization, endotoxin-removal chemistry and high-capacity column options for transfection-grade DNA. |
| Intended Use | Large-scale plasmid DNA purification for transfection, restriction digestion, PCR, sequencing and ligation. |
| Principle / Technology | Alkaline lysis, endotoxin-removal filtration and selective column binding purify plasmid DNA from large bacterial cultures. |
| Detection Method | Downstream DNA analysis and cell transfection. |
| Sample Type | 100-200 mL overnight bacterial culture. |
| Performance Range / Specifications | Approximately 50-minute workflow; yield up to 2.5 mg from 200 mL high-copy culture; 10 reactions; standard and high-capacity column configurations. |
| Specificity | Optimized buffers and filtration remove endotoxin, proteins and other bacterial contaminants while retaining plasmid DNA. |
| Reaction Conditions / Protocol | Harvest culture, resuspend, lyse and neutralize with color indication, clear and filter the lysate, bind DNA to the selected column, wash and elute. |
| Components / Formulation | Resuspension, lysis, neutralization, wash and elution buffers; endotoxin-removal materials; standard or high-capacity plasmid-binding columns. |
| Storage Conditions | Store at 15-25°C. After RNase A is added to the resuspension buffer, store at 2-8°C for up to 6 months. |
| Package Specifications | 10 reactions. |
| Product Form | Large-scale endotoxin-free plasmid DNA extraction kit. |
| Quality Control | Yield, purity, endotoxin level and transfection performance are evaluated during release testing. |
| Key Features | Up to 2.5 mg yield; approximately 50-minute workflow; color-indicated processing; reduced centrifugation; low-endotoxin DNA; multiple column options. |
| Shipping Conditions | Ship at room temperature. |
| Compatibility | Cell transfection and routine molecular biology applications. |
| Application Notes / Precautions | Match column configuration to expected plasmid yield and culture volume; use aseptic RNase handling. |
| Batch-to-Batch Consistency | Yield, purity and endotoxin-removal performance are controlled across batches. |
For research use only, not for clinical use.
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