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| Product Name | Endotoxin-Free Plasmid Mini Plus Extraction Kit |
| Catalog No. | DREK-0088 |
| Description | A spin-column kit for purifying low-endotoxin plasmid DNA from small-to-medium bacterial cultures using alkaline lysis, color-indicated neutralization and selective silica-membrane binding. |
| Intended Use | Plasmid DNA purification for cell transfection, restriction digestion, PCR, sequencing and ligation. |
| Principle / Technology | Alkaline lysis and selective silica-membrane binding with a buffer system designed to remove endotoxin and protein contaminants. |
| Detection Method | Downstream DNA analysis and cell transfection. |
| Sample Type | 5-15 mL overnight bacterial culture. |
| Performance Range / Specifications | Approximately 40-minute workflow; concentration can exceed 1,000 ng/µL from 15 mL high-copy culture; 50 reactions. |
| Specificity | The silica membrane and dedicated buffers selectively recover plasmid DNA while removing endotoxin, proteins and other impurities. |
| Reaction Conditions / Protocol | Harvest culture, resuspend, lyse and neutralize with visual color indication, clarify, bind to the column, wash and elute. |
| Components / Formulation | Resuspension, lysis, neutralization, endotoxin-removal, wash and elution buffers; silica spin columns and collection tubes. |
| Storage Conditions | Store at 15-25°C. After RNase A is added to the resuspension buffer, store at 2-8°C for up to 6 months. |
| Package Specifications | 50 reactions. |
| Product Form | Endotoxin-free plasmid DNA spin-column kit. |
| Quality Control | Yield, purity, endotoxin removal and transfection performance are evaluated during release testing. |
| Key Features | Reduced centrifugation; approximately 40-minute workflow; visual neutralization indicator; high-concentration DNA; low endotoxin. |
| Shipping Conditions | Ship at room temperature. |
| Compatibility | Cell transfection and routine molecular biology applications. |
| Application Notes / Precautions | Culture volume and buffer quantities should be adjusted for low-copy or large plasmids; avoid column overload. |
| Batch-to-Batch Consistency | Yield, purity and endotoxin-removal performance are controlled across batches. |
For research use only, not for clinical use.
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