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| Product Name | Endotoxin-Free Plasmid Midi Extraction Kit |
| Catalog No. | DREK-0089 |
| Description | A medium-scale spin-column kit for isolating low-endotoxin plasmid DNA from bacterial cultures. Color-indicated neutralization helps confirm complete processing before column purification. |
| Intended Use | Plasmid DNA purification for cell transfection, restriction digestion, PCR, sequencing and ligation. |
| Principle / Technology | Optimized alkaline lysis followed by selective silica-membrane binding and endotoxin-removal washes. |
| Detection Method | Downstream DNA analysis and cell transfection. |
| Sample Type | 15-50 mL overnight bacterial culture. |
| Performance Range / Specifications | Yield up to 300 µg from 50 mL high-copy culture; endotoxin residue ≤0.1 EU/µg for high-copy plasmids; 10 reactions. |
| Specificity | Dedicated binding and wash buffers remove endotoxin, proteins and genomic DNA while enriching plasmid DNA. |
| Reaction Conditions / Protocol | Harvest culture, resuspend, lyse and neutralize with color indication, clarify, bind plasmid DNA to the column, wash and elute. |
| Components / Formulation | Resuspension, lysis, neutralization, endotoxin-removal, wash and elution buffers; midi spin columns and collection tubes. |
| Storage Conditions | Store at 15-25°C. |
| Package Specifications | 10 reactions. |
| Product Form | Medium-scale endotoxin-free plasmid DNA extraction kit. |
| Quality Control | Yield, purity, endotoxin level and transfection performance are evaluated during release testing. |
| Key Features | Up to 300 µg yield; endotoxin ≤0.1 EU/µg; visual processing indicator; transfection-compatible DNA. |
| Shipping Conditions | Ship at room temperature. |
| Compatibility | Cell transfection and routine molecular biology applications. |
| Application Notes / Precautions | For low-copy or >10 kb plasmids, increase culture input and prewarm the elution buffer as appropriate. |
| Batch-to-Batch Consistency | Yield, purity and endotoxin-removal performance are controlled across batches. |
For research use only, not for clinical use.
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