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| Product Name | Electrophoresis Tank Cleaning Solution, Protein and Buffer Residue Remover |
| Catalog No. | ATRCS-0027 |
| Description | Formulated cleaning agent for polyacrylamide and agarose gel electrophoresis tanks to remove protein deposits, buffer salt crusts, Coomassie and silver stain residues, and polymerized acrylamide fragments that accumulate on electrodes, gaskets, and tank walls. The non-foaming, mildly acidic formulation dissolves protein precipitates and mineral scale without corroding platinum wire electrodes or cracking acrylic tank bodies. Routine use maintains consistent electrophoretic current and prevents gel-to-gel contamination. |
| Intended Use | Regular cleaning and maintenance of vertical and horizontal electrophoresis tanks, gel casting stands, combs, and electrode assemblies used in protein and nucleic acid electrophoresis. |
| Principle / Technology | Acid-catalyzed hydrolysis of protein deposits coupled with chelation of divalent cations from buffer salts; surfactant action removes hydrophobic dye residues from tank surfaces. |
| Detection Method | Fill tank with working solution; soak 30-60 minutes; scrub gently with soft brush if needed; rinse thoroughly with deionized water; air dry or towel dry. |
| Sample Type | Electrophoresis tanks, gel cassettes, combs, gaskets, electrode assemblies. |
| Performance Range / Specifications | Removes ≥98% of Coomassie Blue stain residue and ≥95% of buffer salt deposits after 30-minute soak at room temperature. |
| Sensitivity / LOD | Visual inspection for stain residues using white background illumination; conductivity measurement of rinse water to verify salt removal. |
| Specificity | Targets protein-stain complexes, buffer salt precipitates, and polymerized acrylamide debris; does not attack acrylic (PMMA) or polycarbonate tank materials. |
| Reaction Conditions / Protocol | Soak at room temperature for 30-60 minutes; for heavy deposits, extend soak to 2 hours or use at 40 °C. |
| Components / Formulation | Citric acid, EDTA disodium salt, nonionic surfactant, preservative, deionized water. |
| Storage Conditions | Store at room temperature (15-25 °C) in original container; keep tightly closed. |
| Shelf Life | 24 months from date of manufacture. |
| Package Specifications | 500 mL, 1 L, 5 L bottles. |
| Product Form | Colorless to pale yellow liquid concentrate. |
| Quality Control | Each lot tested for effective removal of standardized Coomassie-stained protein spots and conductivity reduction in rinse water. |
| Key Features | Non-foaming formulation; safe for platinum electrodes; leaves no fluorescent residue that interferes with gel imaging; biodegradable within 28 days; pleasant citrus scent. |
| Purity | Citric acid USP grade; EDTA ≥99% purity; heavy metals <5 ppm. |
| Concentration | Dilute 1:10 to 1:20 with deionized water depending on soiling level. |
| Activity / Unit Definition | Protein removal efficiency ≥98% based on residual Coomassie absorbance after treatment. |
| Molecular Weight | Not applicable — formulated cleaning solution. |
| Source / Origin | Synthetic and natural-derived ingredients from analytical grade raw materials. |
| pH Range / Optimal pH | Working solution pH 3.5-4.5; concentrate pH 2.0-2.5. |
| Shipping Conditions | Ambient temperature; protect from freezing. |
| Expiration Date / Stability | 24 months at recommended storage; working solution stable for 7 days after dilution. |
| Regulatory / Compliance | For laboratory use; complies with general laboratory safety and hygiene standards. |
| Compatibility | Compatible with acrylic (PMMA), polycarbonate, PVC, silicone rubber, and platinum electrodes. Test on a small area before use on polypropylene tanks. Not recommended for aluminum electrode assemblies. |
| Recommended Buffer System | Citrate-based acidic buffering for controlled protein hydrolysis. |
| Application Notes / Precautions | Do not use undiluted on acrylic surfaces for extended periods. After cleaning, fill tank with deionized water and run a blank electrophoresis gel to verify current consistency. Replace cleaning solution when it becomes visibly discolored. Avoid mixing with bleach-based cleaners. |
| Batch-to-Batch Consistency | pH of working solution within ±0.3 units; cleaning efficacy within ±5% of reference lot. |
For research use only, not for clinical use.
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