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| Product Name | DNA Marker 5000 |
| Catalog No. | NATR-HMM-0157 |
| Description | A ready-to-load double-stranded DNA marker containing defined plasmid-derived fragments and blue loading buffer for routine agarose gel analysis. |
| Intended Use | DNA molecular size estimation during agarose gel electrophoresis. |
| Principle / Technology | Defined double-stranded DNA fragments provide reference migration positions for estimating DNA fragment size. |
| Detection Method | Agarose gel electrophoresis followed by nucleic acid staining and visualization. |
| Sample Type | DNA electrophoresis samples. |
| Performance Range / Specifications | The 1,000 bp fragment is supplied as an enhanced reference band. |
| Components / Formulation | Purified plasmid-derived double-stranded DNA fragments premixed with blue loading buffer. |
| Storage Conditions | Store at −20°C. After thawing, keep at 4°C and avoid repeated freeze-thaw cycles. |
| Package Specifications | 50 reactions; 100 reactions. |
| Product Form | Ready-to-load DNA molecular weight marker. |
| Key Features | Clear and compact bands; accurate relative band mass; direct-loading format; stable at room temperature for up to 6 months. |
| Concentration | 1,000 bp band: 100 ng per 5 μL; all other bands: 50 ng per 5 μL. |
| Expiration Date / Stability | Reported to remain stable at room temperature for 6 months. |
| Compatibility | Designed for agarose gel electrophoresis; not recommended for polyacrylamide gel electrophoresis. |
| Recommended Buffer System | 1× TAE buffer with 0.8–2.0% agarose gel. |
| Application Notes / Precautions | A voltage gradient of 4–10 V/cm is recommended. Use 5 μL per lane for the stated band masses, and avoid nuclease contamination and repeated freeze-thaw cycles. |
For research use only, not for clinical use.
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