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DNA Marker 15000

Cat.No: NATR-HMM-0158 Datasheet

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Product Name DNA Marker 15000
Catalog No. NATR-HMM-0158
Description A ready-to-load double-stranded DNA marker for estimating larger DNA fragments during agarose gel electrophoresis.
Intended Use DNA molecular size estimation during agarose gel electrophoresis.
Principle / Technology Defined double-stranded DNA fragments provide reference migration positions for estimating DNA fragment size.
Detection Method Agarose gel electrophoresis followed by nucleic acid staining and visualization.
Sample Type DNA electrophoresis samples.
Components / Formulation Purified plasmid-derived double-stranded DNA fragments premixed with blue loading buffer.
Storage Conditions Store at −20°C. After thawing, keep at 4°C and avoid repeated freeze-thaw cycles.
Package Specifications 50 reactions; 100 reactions.
Product Form Ready-to-load DNA molecular weight marker.
Key Features Clear and compact bands; accurate relative band mass; direct-loading format; stable at room temperature for up to 6 months.
Concentration Each band: 50 ng per 5 μL.
Expiration Date / Stability Reported to remain stable at room temperature for 6 months.
Compatibility Designed for agarose gel electrophoresis; not recommended for polyacrylamide gel electrophoresis.
Recommended Buffer System 1× TAE buffer with 0.8–2.0% agarose gel.
Application Notes / Precautions A voltage gradient of 4–10 V/cm is recommended. Use 5 μL per lane for the stated band masses, and avoid nuclease contamination and repeated freeze-thaw cycles.

For research use only, not for clinical use.

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