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| Product Name | Direct Cell Two-Step SYBR RT-qPCR Kit |
| Catalog No. | NATR-HMM-0204 |
| Description | A complete two-step dye-based RT-qPCR kit for direct analysis of cultured cells without conventional RNA extraction. |
| Intended Use | High-throughput cell-based drug screening and gene-expression analysis. |
| Principle / Technology | Cells are lysed directly in culture plates, genomic DNA is removed, RNA is reverse transcribed, and cDNA is quantified by dye-based real-time PCR. |
| Detection Method | SYBR Green dye-based two-step RT-qPCR. |
| Sample Type | 10 to 10⁶ cultured adherent or suspension cells. |
| Performance Range / Specifications | Complete cell-to-result workflow in approximately 65–67 minutes; compatible with 384-, 96-, 24-, 12- and 6-well cell-culture plates. |
| Sensitivity / LOD | Supports sensitive detection across a broad cell-input range. |
| Specificity | Optimized reverse-transcription and dye-based qPCR chemistries support reliable analysis of low-expression targets. |
| Reaction Conditions / Protocol | Perform 7–9 minute in-plate lysis and genomic-DNA removal, synthesize first-strand cDNA, then run dye-based qPCR. |
| Components / Formulation | Cell-lysis/genomic-DNA removal module, first-strand cDNA synthesis module and SYBR qPCR master mix. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions; 500 reactions. |
| Product Form | Complete two-step direct-cell dye-based RT-qPCR kit. |
| Quality Control | Cell-number compatibility, cell-type compatibility and dye-based amplification performance are evaluated across multiple culture formats. |
| Key Features | Extraction-free workflow; approximately 65–67 minute analysis; broad cell compatibility; multiple plate formats; dye-based quantitative detection. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with common dye-based real-time PCR instruments and 384/96/24/12/6-well cell-culture formats. |
| Application Notes / Precautions | Ensure thorough mixing during lysis and stop steps and avoid excessive cell input that may inhibit reverse transcription or amplification. |
For research use only, not for clinical use.
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