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| Product Name | Direct Cell Two-Step Probe RT-qPCR Kit |
| Catalog No. | NATR-HMM-0203 |
| Description | A complete two-step probe RT-qPCR workflow that performs in-plate cell lysis, genomic-DNA removal, first-strand cDNA synthesis and sensitive probe qPCR. |
| Intended Use | High-throughput gene-expression analysis and in vitro cell-based drug screening. |
| Principle / Technology | Cultured cells are lysed without RNA purification, converted to first-strand cDNA and quantified using a sensitive probe-based qPCR system. |
| Detection Method | Fluorescent probe-based two-step RT-qPCR. |
| Sample Type | 10 to 10⁶ cultured adherent or suspension cells. |
| Performance Range / Specifications | Complete cell-to-result workflow in approximately 62–65 minutes; compatible with 384-, 96-, 24-, 12- and 6-well plates; reported amplification efficiency 90–110% with R² >0.99 across 10 to 10⁶ cells. |
| Sensitivity / LOD | Optimized for sensitive detection from low cell numbers and low-expression targets. |
| Specificity | Probe-based detection and optimized hot-start amplification support target-specific quantification. |
| Reaction Conditions / Protocol | Perform 7–9 minute in-plate lysis and genomic-DNA removal, synthesize first-strand cDNA, then run probe-based qPCR. |
| Components / Formulation | Cell-lysis/genomic-DNA removal module, first-strand cDNA synthesis module and probe qPCR master mix. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions; 500 reactions. |
| Product Form | Complete two-step direct-cell probe RT-qPCR kit. |
| Quality Control | Workflow linearity, amplification efficiency, cell-number compatibility and probe-qPCR performance are evaluated across multiple culture formats. |
| Key Features | Extraction-free workflow; high sensitivity; broad cell compatibility; high-throughput plate support; complete two-step probe RT-qPCR solution. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with common probe-based real-time PCR instruments and 384/96/24/12/6-well cell-culture formats. |
| Application Notes / Precautions | Ensure complete mixing during lysis and stop steps. Avoid excessive cell input that may inhibit amplification. |
For research use only, not for clinical use.
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