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| Product Name | Direct Cell One-Step Probe RT-qPCR Kit |
| Catalog No. | NATR-HMM-0205 |
| Description | An extraction-free one-step probe RT-qPCR system that combines direct cell lysis, genomic-DNA removal, reverse transcription and probe amplification. |
| Intended Use | Rapid high-throughput gene-expression analysis and cell-based drug screening. |
| Principle / Technology | RNA-containing cell lysate is prepared directly in culture plates and analyzed in a single reverse-transcription and probe-qPCR reaction. |
| Detection Method | Fluorescent probe-based one-step RT-qPCR. |
| Sample Type | 10 to 10⁶ cultured adherent or suspension cells. |
| Performance Range / Specifications | Complete cell-to-result workflow in approximately 37 minutes; compatible with 384-, 96-, 24-, 12- and 6-well cell-culture plates. |
| Sensitivity / LOD | Optimized one-step chemistry supports sensitive detection and reduces missed low-expression targets. |
| Specificity | Probe detection and hot-start amplification support specific quantification and multiplex target analysis. |
| Reaction Conditions / Protocol | Perform 7–9 minute in-plate lysis and genomic-DNA removal, then add lysate directly to the one-step probe RT-qPCR reaction. |
| Components / Formulation | Cell-lysis/genomic-DNA removal module and one-step probe RT-qPCR master mix. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions; 500 reactions. |
| Product Form | Complete direct-cell one-step probe RT-qPCR kit. |
| Quality Control | Cell-input range, lysis compatibility, one-step amplification sensitivity and probe-detection performance are evaluated. |
| Key Features | Approximately 37-minute workflow; no RNA extraction; broad cell compatibility; high-throughput format; multiplex probe compatibility. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with common probe-based real-time PCR instruments and multiple cell-culture plate formats. |
| Application Notes / Precautions | Use the recommended cell-input range and mix lysis and stop reagents thoroughly to avoid incomplete lysis, genomic-DNA carryover or amplification inhibition. |
For research use only, not for clinical use.
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