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| Product Name | Direct Cell Lysis Kit for RT-qPCR |
| Catalog No. | NATR-HMM-0201 |
| Description | A rapid extraction-free cell-lysis system that removes genomic DNA and releases RNA directly in culture plates for downstream gene-expression analysis. |
| Intended Use | In vitro cell-based drug screening; cellular RNA expression analysis; direct RT-PCR and RT-qPCR sample preparation. |
| Principle / Technology | Cultured cells are lysed in situ, genomic DNA is removed and the resulting RNA-containing lysate is used directly in reverse-transcription or one-step RT-qPCR reactions. |
| Sample Type | 10 to 10⁶ cultured adherent or suspension cells. |
| Performance Range / Specifications | Two-step workflow completed in approximately 7–9 minutes; compatible with 384-, 96-, 24-, 12- and 6-well culture plates. |
| Reaction Conditions / Protocol | Add lysis working solution directly to cultured cells, mix thoroughly, incubate for the recommended lysis period, add stop buffer and proceed promptly to downstream analysis. |
| Components / Formulation | Cell lysis and genomic-DNA removal reagents with stop buffer. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions; 500 reactions. |
| Product Form | Direct cell lysis reagent kit. |
| Key Features | No column purification, transfer or centrifugation; 7–9 minute sample preparation; broad cell-number range; in-plate processing; high-throughput compatibility. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Lysate can be used for reverse transcription and one-step RT-qPCR with dye-based or probe-based detection. |
| Application Notes / Precautions | Do not leave lysate at room temperature for more than 20 minutes or on ice for more than 2 hours. For longer storage, freeze lysate at −80°C. Use fewer than 10⁶ cells per reaction to limit endogenous RNase and inhibitor effects. |
For research use only, not for clinical use.
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