| Product Name | Direct Bilirubin (DBIL) Content Assay Kit (Visible Spectrophotometers) |
| Catalog No. | BBITK-HMM-0027 |
| Description | Direct bilirubin (DBIL), also known as conjugated bilirubin, is formed when indirect bilirubin enters the liver and combines with glucuronic acid under the action of glucuronosyltransferase in the liver. An increase in direct bilirubin is of great significance for the clinical diagnosis of obstructive jaundice, hepatocellular jaundice, liver cancer, pancreatic head cancer, cholestasis, cholangiocarcinoma, etc. |
| Testing Equipment | Visible Spectrophotometers |
| Matching | 1 mL glass cuvette (d=10 mm) |
| Number of Testable Samples | 48 Samples |
| Estimated Measurement Time | 2 h (48 Samples) |
| Storage | Store at 4°C |
| Self-contained Reagents | / |
| Detection Principle | Direct bilirubin can be oxidized by sodium nitrite to form biliverdin, bilirubin has a characteristic absorption peak at 450 nm, and the content of direct bilirubin can be quantified by the change of absorbance value. |
| Detection Methods | Sodium Nitrite Oxidation Method |
| Detection Wavelength | 450 nm |
| Signal Response | Decremental |
| Note | Bilirubin is easily decomposed in the presence of light, the operation should be carried out under the condition of avoiding light; If ΔA is greater than 0.5, it is recommended to dilute the sample to be measured appropriately and then carry out the determination, and the calculation should be modified accordingly. |
Direct bilirubin (DBIL), also known as conjugated bilirubin, is a key intermediate in bilirubin metabolism and a critical target for hepatobiliary system research. Its biosynthesis involves a precise enzymatic reaction: indirect bilirubin (unconjugated bilirubin), a byproduct of erythrocyte degradation, enters hepatocytes and conjugates with glucuronic acid under the catalysis of glucuronosyltransferase. This conjugation transforms the lipid-soluble indirect bilirubin into water-soluble direct bilirubin, which is then transported to the biliary system for eventual excretion.
In scientific research, the quantitative analysis of direct bilirubin serves as a fundamental tool for exploring liver function mechanisms, bilirubin metabolism pathways, and the pathogenesis of hepatobiliary-related disorders. Research focusing on liver cell physiology, drug-induced liver injury, and genetic defects in bilirubin metabolism all rely on accurate direct bilirubin detection to validate hypotheses and draw conclusions. For example:
Studies on hepatocellular metabolism: Investigating how liver cells process and excrete bilirubin under normal and pathological conditions, which helps clarify the molecular mechanisms of liver function regulation.
Drug development research: Evaluating the impact of candidate drugs on liver metabolism by monitoring direct bilirubin levels in experimental models, ensuring drug safety and efficacy in preclinical stages.
Disease mechanism exploration: Exploring the role of abnormal direct bilirubin accumulation in the development of conditions such as cholestasis, liver fibrosis, and hepatocellular carcinoma in animal models or in vitro cell cultures.
Over the years, multiple detection methodologies have been developed for direct bilirubin, including diazo coupling, oxidase-based assays, high-performance liquid chromatography (HPLC), and chemical oxidation methods. Among these, the sodium nitrite oxidation method integrated with visible spectrophotometry stands out for its balance of simplicity, cost-efficiency, and reliability—making it a preferred choice for academic research laboratories, biotech R&D teams, and pharmaceutical research institutions. Our Direct Bilirubin (DBIL) Content Assay Kit leverages this well-established method to provide researchers with a consistent and accessible tool for quantitative DBIL analysis in research samples.
The demand for high-quality direct bilirubin assay kits in the research community continues to grow, driven by the expanding focus on liver biology, metabolic disorders, and drug discovery. As research becomes more specialized—from in vitro hepatocyte culture studies to in vivo animal model experiments—there is an increasing need for assay tools that are adaptable, accurate, and tailored to research-specific workflows. Our kit is designed to meet these needs, supporting a wide range of research applications while maintaining the precision required for scientific rigor.
Validated Detection Principle: Employs the sodium nitrite oxidation method, where direct bilirubin is oxidized to biliverdin. With bilirubin’s characteristic absorption peak at 450 nm, quantitative analysis is achieved by measuring absorbance changes at this wavelength—an approach widely recognized and validated in academic research for its specificity to direct bilirubin.
Standard Equipment Compatibility: Optimized for use with visible spectrophotometers, a staple instrument in most research laboratories. This eliminates the need for specialized or high-cost detection equipment, ensuring seamless integration into existing research workflows.
Standard Cuvette Matching: Compatible with 1 mL glass cuvettes (10 mm light path), a universally available lab consumable. Consistent optical path conditions enhance the reproducibility of absorbance measurements across different experiments.
Research-Scalable Throughput: Each kit can process up to 48 samples, aligning with typical batch sizes in research settings—from small-scale preliminary experiments to medium-volume data collection, balancing efficiency and reagent utilization.
Controllable Experimental Timing: Requires approximately 2 hours to complete 48 sample tests, allowing researchers to plan experimental schedules effectively and integrate DBIL analysis into multi-step research protocols.
Convenient Storage Conditions: Stable when stored at 4°C, a standard refrigeration temperature in research labs. No need for ultra-low temperature storage, simplifying inventory management and reducing reagent degradation risks.
Light-Sensitivity Guidance: Includes clear instructions to perform all operations under light-avoiding conditions, as bilirubin is photosensitive. This detail preserves sample integrity and prevents inaccurate results caused by bilirubin decomposition.
Flexible Sample Concentration Adaptability: Provides guidance for high-concentration samples: if ΔA exceeds 0.5, samples can be appropriately diluted (with calculation adjustments) to ensure accurate quantification. This expands the kit’s utility for samples with variable DBIL levels in research models.
Research-Grade Accuracy: The sodium nitrite oxidation method minimizes interference from other biological components (e.g., indirect bilirubin, proteins, lipids) in research samples. This specificity ensures that results accurately reflect DBIL concentrations, supporting reliable data interpretation for research publications and project milestones.
Consistent Reproducibility: Manufactured with strict quality control standards, ensuring batch-to-batch consistency. Low variability in repeated measurements enhances the statistical power of research data, critical for validating experimental findings.
Cost-Effective for Research Budgets: Offers a lower cost-per-test compared to advanced methods like HPLC, without compromising accuracy. Ideal for labs with limited funding or high sample volumes, reducing the overall cost of research projects.
User-Friendly Protocol: Requires no complex sample preprocessing (beyond optional dilution for high-concentration samples). The straightforward workflow reduces the learning curve for researchers, saving time on training and experimental setup.
Broad Research Application Scope: Suitable for various research samples, including animal serum, plasma, hepatocyte culture supernatants, and tissue homogenates. Supports diverse research areas such as liver biology, metabolic disease research, drug toxicity testing, and bilirubin metabolism studies.
Dedicated Research Support: Provides professional technical assistance for research-specific challenges—whether troubleshooting experimental procedures, optimizing sample handling, or interpreting results. Our team of experts understands research workflows and can offer tailored guidance to ensure smooth experiment execution.
For research use only, not for clinical use.
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