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| Product Name | Digital Droplet PCR Supermix (EvaGreen-Based) |
| Catalog No. | NATR-HMM-0147 |
| Description | A specialized PCR master mix formulated for droplet digital PCR applications using EvaGreen intercalating dye detection. The supermix contains all components required for droplet generation, thermal cycling, and fluorescence reading on droplet digital PCR platforms. The optimized buffer system ensures uniform droplet stability through thermal cycling while the hot-start polymerase formulation prevents non-specific amplification during droplet generation steps. |
| Intended Use | Absolute quantification of target nucleic acid sequences by droplet digital PCR without the need for standard curves. |
| Principle / Technology | Droplet digital PCR with EvaGreen fluorescent intercalating dye |
| Detection Method | Droplet-based fluorescence endpoint measurement |
| Sample Type | Purified DNA including genomic DNA, cDNA, and plasmid DNA; compatible with both extracted and crude lysate samples |
| Performance Range / Specifications | Dynamic range: 1–100,000 copies per 20 µL reaction; droplet count: ≥10,000 accepted droplets per well |
| Sensitivity / LOD | Limit of detection: 0.5 copies per µL; limit of quantification: 2 copies per µL |
| Specificity | EvaGreen dye binds all double-stranded DNA; target specificity conferred by primer design; melt curve analysis optional after amplification |
| Reaction Conditions / Protocol | Droplet generation: follow ddPCR system instructions; thermal cycling: 95 °C 10 min, 40×(94 °C 30 sec, 60 °C 60 sec), 98 °C 10 min; ramp rate: 2 °C/sec |
| Components / Formulation | 2× ddPCR EvaGreen Supermix, droplet generation oil, nuclease-free water |
| Storage Conditions | –20 °C, protected from light; avoid repeated freeze-thaw cycles |
| Shelf Life | 12 months at –20 °C |
| Package Specifications | 5 mL supermix (sufficient for 200 × 25 µL reactions); separate oil provided |
| Product Form | Liquid, ready-to-use 2× master mix |
| Quality Control | Each lot tested on a ddPCR system with reference DNA template; droplet count, amplitude separation, and copy number linearity verified |
| Key Features | EvaGreen dye chemistry eliminates the need for target-specific probes, reducing assay design complexity |
| Purity | DNase/RNase free; PCR inhibitor free; A260/A280 ≥1.8 for nucleic acid products |
| Concentration | As specified on product label; enzyme units per µL as stated |
| Activity / Unit Definition | Polymerase activity defined as nmol dNTP incorporated per 30 min per unit; other activities per product specification |
| Molecular Weight | As specified per DNA markers or protein components |
| Source / Origin | Recombinant enzymes expressed in E. coli; synthetic oligonucleotides; ultrapure reagents |
| pH Range / Optimal pH | pH 7.5–8.5 for PCR; pH 8.0 for most molecular biology applications |
| Shipping Conditions | Cold packs or dry ice for enzymes; ambient for buffers and DNA markers |
| Expiration Date / Stability | 12–24 months at –20 °C for enzymes; 12–24 months at room temperature for buffers |
| Regulatory / Compliance | Research use; ISO 13485 manufacturing for select products |
| Compatibility | Compatible with standard thermal cyclers, real-time PCR instruments, and gel electrophoresis systems |
| Recommended Buffer System | Tris-HCl, KCl, MgCl₂-based reaction buffer; TE for nucleic acid storage |
| Application Notes / Precautions | Use dedicated PCR workspace; aliquot enzymes to avoid contamination; monitor no-template controls |
| Batch-to-Batch Consistency | Enzyme activity within ±15% of reference lot; PCR performance verified with control template |
For research use only, not for clinical use.
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