- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | DEAE-Dextran Transfection Reagent, Soluble Form, for COS-7 Cells |
| Catalog No. | ATR-CTR-0028 |
| Description | Ready-to-use DEAE-dextran (diethylaminoethyl-dextran) transfection reagent solution for efficient transient nucleic acid delivery into COS-7, CV-1, BSC-1, and other African green monkey kidney-derived cell lines. DEAE-dextran is a cationic polymer that complexes with negatively charged DNA to form positively charged particles that bind to the cell surface via electrostatic interactions with anionic proteoglycans. Uptake occurs through endocytosis, enhanced by a brief DMSO or glycerol shock step that permeabilizes endosomal membranes to release DNA into the cytoplasm. This method is particularly efficient for COS cell lines expressing SV40 large T-antigen, enabling episomal replication of SV40 origin-containing plasmids for very high expression levels. |
| Intended Use | High-efficiency transient transfection of COS-7 and related cell lines for recombinant protein expression, SV40 origin-containing plasmid amplification, virus production, and functional assays in adherent cell culture format. |
| Principle / Technology | Cationic DEAE-dextran polymer electrostatically binds and condenses negatively charged plasmid DNA; positively charged DNA-polymer complex binds cell surface proteoglycans; endocytosis of complex; DMSO/glycerol shock permeabilizes endosomes releasing DNA; SV40 T-antigen in COS cells amplifies SV40 ori plasmids to high copy number. |
| Detection Method | Mix DNA (2-10 µg) with DEAE-dextran solution in serum-free medium or PBS; incubate 15-30 min at RT; wash cells with PBS; add DNA/Dextran mix to cells; incubate 30 min-4 hours at 37 °C; remove mix; apply DMSO or glycerol shock (10% DMSO in PBS, 2 min); wash; add complete growth medium; assay 24-72 hours post-transfection. |
| Sample Type | Adherent COS-7, COS-1, CV-1, BSC-1, and other SV40 T-antigen expressing cell lines. |
| Performance Range / Specifications | Transfection efficiency: 40-80% for COS-7 cells with EGFP reporter; expression levels: 1-10 µg recombinant protein per 10^6 cells for SV40 ori-containing plasmids. |
| Sensitivity / LOD | Transgene expression detectable within 12-18 hours; peak expression at 48-72 hours post-transfection. |
| Specificity | Most efficient in COS cell lines expressing SV40 large T-antigen; limited efficiency in cells lacking T-antigen; DMSO/glycerol shock is essential for DNA release from endosomes. |
| Reaction Conditions / Protocol | DEAE-dextran concentration: 100-500 µg/mL; DNA concentration: 1-5 µg/mL; incubation: 30 min to 4 hours; DMSO shock: 10% in PBS, 2 min at RT. |
| Components / Formulation | DEAE-dextran solution (10 mg/mL in PBS, sterile-filtered), DMSO shock solution (sterile), transfection protocol optimized for COS-7 cells. |
| Storage Conditions | Store DEAE-dextran at 2-8 °C; DMSO at room temperature; protect from light. |
| Shelf Life | 12 months from date of manufacture. |
| Package Specifications | 1 mL (10 mg DEAE-dextran), 5 mL (50 mg), for ~50-500 transfections. |
| Product Form | DEAE-dextran: clear, colorless to pale yellow viscous solution; DMSO: clear liquid. |
| Quality Control | Each lot tested for DEAE-dextran concentration, molecular weight (average 500,000 Da), sterility, endotoxin (<0.1 EU/mL), and transfection efficiency in COS-7 cells (EGFP >50% positive). |
| Key Features | Ready-to-use DEAE-dextran solution; optimized for COS cell lines; DMSO shock reagent included; enables SV40 ori plasmid amplification; cost-effective; minimal DNA required (2-5 µg per 100 mm dish); no liposome formation required. |
| Purity | DEAE-dextran content within specification; substitution degree 1 nitrogen per 2-5 glucose units; endotoxin <0.1 EU/mL. |
| Concentration | 10 mg/mL in PBS; typical working concentration 100-500 µg/mL. |
| Activity / Unit Definition | DNA binding capacity: ~1 µg DNA per 10 µg DEAE-dextran under optimal ionic conditions. |
| Molecular Weight | DEAE-dextran: average 500,000 Da (dextran backbone ~500 kDa with DEAE substitution). |
| Source / Origin | Dextran from Leuconostoc mesenteroides fermentation, chemically modified with diethylaminoethyl chloride. |
| pH Range / Optimal pH | pH 7.0-7.4. |
| Shipping Conditions | Ambient or cold pack. |
| Expiration Date / Stability | 12 months at 2-8 °C; after opening, use within 6 months. |
| Regulatory / Compliance | For research use only; not for therapeutic or diagnostic applications. |
| Compatibility | Most efficient in COS-7 cells. Also works with CV-1, BSC-1, HeLa, NIH/3T3, and CHO with reduced efficiency. DEAE-dextran is toxic to some cell types with prolonged exposure — optimize incubation time. Serum-free medium required during transfection step as serum proteins compete for DNA binding. |
| Recommended Buffer System | PBS without Ca2+/Mg2+ for transfection mixture; DMSO shock in PBS. |
| Application Notes / Precautions | Optimize DEAE-dextran concentration for each cell line (100-500 µg/mL range). DMSO shock is critical — it is toxic if exposure exceeds 2 minutes; work quickly and have wash medium ready. Aspirate DMSO completely after shock step and wash cells twice. For chloroquine treatment: add 100 µM chloroquine during transfection incubation to inhibit lysosomal degradation. SV40 ori-containing plasmids amplify to very high copy number in COS-7 cells — 1-10 µg protein per 10^6 cells achievable. DEAE-dextran is not suitable for stable transfection. |
| Batch-to-Batch Consistency | DEAE-dextran concentration within ±10% of specification; transfection efficiency in COS-7 standard assay within ±15% of reference lot. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
