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| Product Name | CRISPR-Cas9 Ribonucleoprotein Delivery Kit, Lipid-Based |
| Catalog No. | ATR-CTR-0010 |
| Description | A complete kit containing Cas9 nuclease, synthetic guide RNA, and optimized delivery reagent for the transient delivery of pre-assembled CRISPR-Cas9 ribonucleoprotein complexes into mammalian cells for genome editing. |
| Intended Use | Transient genome editing in mammalian cells with reduced off-target effects compared to plasmid-based CRISPR delivery; gene knockout generation; homology-directed repair template co-delivery for precise genome modifications. |
| Principle / Technology | Pre-complexed Cas9 protein and synthetic sgRNA form stable RNPs that are delivered via cationic lipid nanoparticles; RNPs are rapidly degraded in the cell, limiting the editing window and reducing off-target cutting compared to constitutively expressed Cas9. |
| Detection Method | Target gene editing efficiency by T7E1 assay, Sanger sequencing decomposition (TIDE analysis), or targeted amplicon NGS |
| Sample Type | Adherent and suspension mammalian cell lines: HEK293, HeLa, Jurkat, K562, U2OS, A549, iPSCs |
| Performance Range / Specifications | Indel frequency 30–90% at target loci depending on sgRNA efficiency; off-target editing <1% for validated high-specificity sgRNAs |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Resuspend Cas9 protein and sgRNA in complexing buffer; incubate 10 min at room temperature to form RNP; dilute lipid reagent; combine RNP and reagent; incubate 10 min; add to cells; assay editing at 48–72 hours. |
| Components / Formulation | SpyFi Cas9 Nuclease (10 µg), synthetic sgRNA (custom sequence), Cas9 complexing buffer, CRISPRMAX lipid transfection reagent, positive control sgRNA targeting HPRT gene |
| Storage Conditions | -20°C (Cas9 protein, sgRNA); 2–8°C (transfection reagent) |
| Shelf Life | 12 months at -20°C (Cas9); 6 months at 2–8°C (reagent) |
| Package Specifications | Kit for 24 reactions (24-well plate), 96 reactions |
| Product Form | Protein, RNA oligonucleotides, lipid reagent, buffers |
| Quality Control | Cas9 nuclease activity by in vitro cleavage assay, transfection efficiency with HPRT control sgRNA in HEK293 cells, sterility of reagent |
| Key Features | Complete RNP delivery kit; reduced off-target editing; transient Cas9 expression eliminates DNA integration risk; validated sgRNA design; rapid editing timeline |
For research use only, not for clinical use.
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