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| Product Name | Conventional Phenol-Guanidinium Total RNA Extraction Reagent |
| Catalog No. | DREK-0106 |
| Description | A conventional phenol/guanidinium reagent with strong lysis and rapid RNase inactivation for total RNA isolation from cultured cells, animal tissues, microorganisms and plant tissues with low secondary-metabolite content. |
| Intended Use | Total RNA isolation from cell and tissue samples for downstream RNA analysis. |
| Principle / Technology | Phenol/guanidinium lysis, chloroform phase separation, aqueous-phase recovery and isopropanol precipitation. |
| Detection Method | Downstream RNA analysis. |
| Sample Type | Cultured cells, animal tissues, microorganisms and simple plant tissues such as seedlings and young leaves. |
| Performance Range / Specifications | Strong sample lysis with rapid protection of RNA integrity; supplied as 100 mL. |
| Specificity | Phase separation partitions RNA into the aqueous phase while reducing DNA and protein carryover. |
| Reaction Conditions / Protocol | Homogenize the sample in the reagent, add chloroform, centrifuge at 2-8°C, collect the aqueous phase, precipitate RNA with isopropanol, wash and dissolve. |
| Components / Formulation | Ready-to-use phenol/guanidinium total RNA extraction reagent. |
| Storage Conditions | Store at 4°C protected from light. |
| Package Specifications | 100 mL. |
| Product Form | Liquid phenol/guanidinium RNA extraction reagent. |
| Quality Control | RNA yield, purity and integrity are assessed using representative cell and tissue samples. |
| Key Features | Strong lysis; broad compatibility with cells, tissues and microorganisms; rapid RNase inactivation; established phase-separation workflow. |
| Compatibility | RT-PCR, qPCR, blotting and other RNA-analysis workflows. |
| Application Notes / Precautions | Use a fume hood and suitable personal protective equipment. Collect the aqueous phase carefully to avoid DNA and protein carryover. |
| Batch-to-Batch Consistency | RNA yield and integrity are controlled across production batches. |
For research use only, not for clinical use.
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