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| Product Name | Complex Tissue and Cell Total RNA Isolation Kit |
| Catalog No. | DREK-0110 |
| Description | A single-column total RNA purification system designed for difficult high-protein, high-fiber and high-fat animal tissues as well as routine animal cells, tissues and plant samples. Optimized lysis and selective silica binding support high RNA yield and purity. |
| Intended Use | Total RNA preparation for RT-PCR, qRT-PCR, Northern blotting, dot blotting, in vitro translation and high-throughput sequencing. |
| Principle / Technology | Optimized chemical lysis followed by selective silica-membrane binding, washing and elution of total RNA. |
| Detection Method | Downstream RNA analysis. |
| Sample Type | Cultured cells; routine animal tissues; muscle, cartilage, fish, shrimp and adipose tissue; compatible plant samples. |
| Performance Range / Specifications | Approximately 10-minute single-sample workflow; enhanced recovery of degraded and short RNA, including 5S RNA; 50 reactions. |
| Reaction Conditions / Protocol | Homogenize and lyse the sample thoroughly, clarify as specified, bind RNA to the silica column, wash and elute. |
| Components / Formulation | Selective silica RNA spin column and optimized lysis reagents. |
| Package Specifications | 50 reactions. |
| Product Form | Spin-column total RNA isolation kit. |
| Key Features | High success rate with complex tissues; rapid workflow; broad sample compatibility; improved recovery of degraded and short RNA. |
| Compatibility | RT-PCR, qRT-PCR, blotting, in vitro translation and sequencing workflows. |
| Application Notes / Precautions | Complete homogenization is essential for fibrous, fatty or protein-rich tissues. Do not exceed the recommended sample input. |
For research use only, not for clinical use.
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