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| Product Name | Column-Based Soil DNA Extraction Kit |
| Catalog No. | DREK-0029 |
| Description | Silica membrane spin column-based kit for genomic DNA extraction from soil and sediment samples. Combines mechanical bead beating with chemical lysis and a specialized inhibitor removal precipitation step to produce PCR-ready DNA with low humic acid carryover. Designed for laboratories preferring column-based workflows over magnetic bead systems for environmental microbiology applications. |
| Intended Use | Genomic DNA extraction from soil, sediment, and compost for 16S rRNA gene sequencing, metagenomics, and qPCR analysis of microbial communities. |
| Principle / Technology | Mechanical lysis (bead beating) + SDS/proteinase K chemical lysis; humic acid precipitation with aluminum ammonium sulfate; DNA binding to silica membrane in high-salt buffer; wash with ethanol-based buffers; elution in low-salt buffer |
| Detection Method | Add 0.5 g soil to bead tube with lysis buffer; bead beat 10 min; add proteinase K, 65 °C 10 min; add aluminum ammonium sulfate, incubate 5 min on ice, centrifuge; transfer supernatant; add binding buffer; load onto spin column; wash 2×; elute in 50-100 µL |
| Sample Type | 0.25-0.5 g soil, sediment, compost; fresh, frozen, or dried |
| Sensitivity / LOD | Yields 1-10 µg DNA per 0.5 g soil depending on biomass; A260/A280 1.6-1.9; PCR-amplifiable without dilution |
| Components / Formulation | Bead beating tubes (0.1 mm + 0.5 mm zirconia beads), lysis buffer with SDS, proteinase K (20 mg/mL), aluminum ammonium sulfate inhibitor precipitation solution, binding buffer, silica membrane spin columns, wash buffer 1, wash buffer 2, elution buffer (Tris 10 mM, pH 8.0), collection tubes |
| Storage Conditions | Proteinase K at -20 °C; remaining components at 15-25 °C |
| Shelf Life | 12 months from manufacture date |
| Package Specifications | 50 preps, 100 preps, 250 preps |
| Product Form | Liquid reagents with bead beating tubes and spin columns |
| Key Features | Proven inhibitor removal; bead beating + chemical lysis; silica membrane spin columns; aluminum ammonium sulfate precipitation for humic acid removal; produces PCR-ready DNA; protocol ~75 min; validated for diverse soil types |
| Purity | A260/A280 1.6-1.9; A260/A230 typically >1.3; PCR amplification successful at 1:5 dilution minimum |
| Concentration | Elution volume and yield as specified per sample type |
| Activity / Unit Definition | DNA/RNA binding capacity per column or per mg beads as specified |
| Molecular Weight | Genomic DNA >50 kb; RNA 0.1–10 kb range as applicable |
| Source / Origin | Silica membrane or magnetic bead technology; recombinant Proteinase K |
| pH Range / Optimal pH | Binding buffer pH 5.0–7.0 for silica-based binding |
| Shipping Conditions | Ambient (most components); proteinase K cold pack |
| Expiration Date / Stability | 12 months; proteinase K 12 months at -20 °C; spin columns do not expire if stored dry at RT |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 |
| Compatibility | Compatible with manual spin columns, vacuum manifolds, and automated liquid handlers |
| Recommended Buffer System | Chaotropic salt binding buffer; ethanol-based wash; low-salt TE or water elution |
| Application Notes / Precautions | Pre-warm elution buffer to improve yield; avoid cross-contamination between samples; change pipette tips between steps |
| Batch-to-Batch Consistency | DNA yield and purity within ±15% of reference lot on standardized blood sample |
For research use only, not for clinical use.
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