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| Product Name | CHO Cell Optimized Transfection Reagent Kit, High-Yield |
| Catalog No. | ATR-CTR-0023 |
| Description | Cationic lipid transfection kit optimized for CHO-K1, CHO-S, CHO-DG44, and CHO-GS knockout cells. Achieves >80% efficiency with plasmid DNA in suspension and adherent cultures. Includes enhancer for extended high-yield expression reaching IgG titers of 100-500 mg/L in transient production. Chemically defined and animal component-free for biopharmaceutical development. |
| Intended Use | High-efficiency CHO transfection for transient protein production; stable cell line generation; antibody and fusion protein expression; glycoengineered protein production. |
| Principle / Technology | Cationic lipid-DNA complexes optimized for CHO membrane composition; enhancer extends expression by reducing cellular stress; optimized for high-density suspension culture |
| Detection Method | For 30 mL CHO-S at 3×10⁶ cells/mL: dilute 30 µg DNA + 60 µL reagent in transfection medium; complex 10 min; add to cells; add enhancer at 4 hr; harvest day 5-7 |
| Sample Type | CHO-K1, CHO-S, CHO-DG44, CHO-GS⁻/⁻ cell lines |
| Reaction Conditions / Protocol | Complex: 10-15 min RT; transfection: 4 hr; enhancer: at 4 hr; expression: 5-7 days |
| Components / Formulation | CHO transfection reagent, CHO transfection medium (serum-free, low-conductivity), CHO enhancer reagent |
| Storage Conditions | 2-8 °C; do not freeze |
| Shelf Life | 12 months from manufacture date |
| Package Specifications | Kit for 50 mL, 250 mL, 1 L culture volumes |
| Product Form | Sterile liquid reagents, ready-to-use |
| Key Features | >80% CHO transfection efficiency; enhancer extends expression to 7 days; IgG titers 100-500 mg/L; compatible with high-density culture (>10×10⁶ cells/mL); chemically defined ACF |
| Purity | All components chemically defined; endotoxin <0.05 EU/mL each; ACF certified |
| Concentration | As specified (typically 1 mg/mL for lipid reagents) |
| Activity / Unit Definition | Transfection efficiency: ≥70% in HEK293 cells with control plasmid at recommended ratio |
| Molecular Weight | Varies by formulation as specified in product documentation |
| Source / Origin | Synthetic cationic lipids and polymers; recombinant viral components where applicable |
| pH Range / Optimal pH | Lipoplex formation optimal at pH 7.0–7.4 |
| Shipping Conditions | Cold pack 2-8 °C; do not freeze reagent |
| Expiration Date / Stability | 12 months at 2-8 °C; enhancer 3 months after opening |
| Regulatory / Compliance | For laboratory and research use only; RUO; ACF; ISO 9001 |
| Compatibility | Compatible with serum-containing and serum-free culture media; check product-specific compatibility |
| Recommended Buffer System | Serum-free medium or PBS for complex formation; complete medium for post-transfection culture |
| Application Notes / Precautions | Optimize reagent-to-DNA ratio per cell type; avoid antibiotic during transfection; monitor cytotoxicity |
| Batch-to-Batch Consistency | Transfection efficiency within ±15% of reference lot on HEK293 standard |
For research use only, not for clinical use.
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