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| Product Name | Cell Culture Incubator Disinfectant, Quaternary Ammonium-Based, Broad Spectrum |
| Catalog No. | ATRCS-0029 |
| Description | Broad-spectrum disinfectant spray specifically formulated for CO2 incubator interior surfaces, water pans, and shelf assemblies. The quaternary ammonium and biguanide dual-active system provides effective disinfection against bacteria, fungi, mycoplasma, and enveloped viruses commonly encountered in cell culture laboratories. The low-residue formula leaves minimal surface film that could outgas and affect cell growth, while the inclusion of a copper-based corrosion inhibitor protects the copper CO2 sensor and stainless steel incubator components from oxidative damage. |
| Intended Use | Routine disinfection of CO2 incubator chambers, water humidification pans, stainless steel shelves, and incubator door gaskets to maintain sterile cell culture conditions and prevent microbial contamination outbreaks. |
| Principle / Technology | Quaternary ammonium and polyhexamethylene biguanide (PHMB) disrupt microbial cytoplasmic membranes; nonionic surfactant removes biofilm matrix; corrosion inhibitors protect metal surfaces. |
| Detection Method | Spray onto surface; wipe with sterile lint-free cloth after 5-minute contact time; rinse water pan with sterile water before refilling. |
| Sample Type | CO2 incubator interior surfaces, water pans, shelves, inner door panels, gaskets. |
| Performance Range / Specifications | ≥5-log reduction of bacteria (including mycoplasma species); ≥4-log reduction of fungi (C. albicans, A. niger); complete inactivation of enveloped viruses after 5 minutes contact. |
| Sensitivity / LOD | Mycoplasma elimination verified by PCR detection post-treatment; residual disinfectant below cell culture cytotoxicity threshold (IC50 >1:100 dilution). |
| Specificity | Broad-spectrum activity against Gram-positive and Gram-negative bacteria, mycoplasma, fungi, and enveloped viruses; limited activity against bacterial spores. |
| Reaction Conditions / Protocol | Spray and leave for 5 minutes contact time; wipe surfaces; for water pans, soak 15 minutes then rinse thoroughly. |
| Components / Formulation | Alkyl dimethyl benzyl ammonium chloride (0.15%), polyhexamethylene biguanide (0.05%), nonionic surfactant, copper corrosion inhibitor, chelating agent, deionized water. |
| Storage Conditions | Store at room temperature (15-25 °C); keep container tightly closed; protect from freezing. |
| Shelf Life | 18 months from date of manufacture. |
| Package Specifications | 500 mL trigger spray bottle, 1 L trigger spray bottle, 5 L jerry can. |
| Product Form | Clear, colorless liquid with mild characteristic odor. |
| Quality Control | Each lot tested for antimicrobial efficacy against S. aureus, E. coli, P. aeruginosa, C. albicans, and A. brasiliensis; cytotoxicity testing on HEK293 and HeLa cell lines. |
| Key Features | Dual-active quaternary ammonium and biguanide formula; mycoplasma-effective; low-residue; copper sensor safe; validated for CO2 incubator use; cell culture cytotoxicity tested. |
| Purity | Active ingredient content within specification ±5%; bacterial endotoxin <0.1 EU/mL; mycoplasma-free certified. |
| Concentration | Ready-to-use; do not dilute for incubator disinfection. |
| Activity / Unit Definition | Antimicrobial activity: ≥5-log10 reduction per EN 13697 surface disinfection test after 5 minutes. |
| Molecular Weight | Not applicable — formulated biocide product. |
| Source / Origin | Synthetic quaternary ammonium and biguanide biocides; pharmaceutical-grade water. |
| pH Range / Optimal pH | pH 6.0-7.0 at 25 °C. |
| Shipping Conditions | Ambient temperature. |
| Expiration Date / Stability | 18 months at recommended storage; after opening, use within 6 months for optimal efficacy. |
| Regulatory / Compliance | For laboratory use only; not registered as a medical device disinfectant. Conforms to laboratory biosafety level 2 (BSL-2) surface decontamination requirements. |
| Compatibility | Compatible with stainless steel (304, 316), copper, anodized aluminum, tempered glass, and silicone gaskets. Prolonged contact with acrylic should be avoided. |
| Recommended Buffer System | Citrate-phosphate buffer for maintaining active biocide efficacy at neutral pH. |
| Application Notes / Precautions | Remove cell culture vessels from incubator before disinfection. Allow surfaces to dry completely before returning cultures; residual moisture can alter incubator humidity control. Change water pan water after each disinfection cycle. Perform incubator disinfection at least monthly, or immediately following any suspected contamination event. Do not autoclave this product. |
| Batch-to-Batch Consistency | Active quaternary ammonium content within ±5% of specification; antimicrobial kill rate within ±0.5 log of reference lot. |
For research use only, not for clinical use.
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