Cell and Tissue miRNA Isolation Kit
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Cell and Tissue miRNA Isolation Kit

Cat.No: DREK-0115 Datasheet

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Product Details Related Products
Product Name Cell and Tissue miRNA Isolation Kit
Catalog No. DREK-0115
Description A spin-column kit for isolating microRNA and other small RNA species from animal cells and tissues. The workflow is designed for efficient small-RNA recovery while reducing high-molecular-weight RNA and genomic DNA carryover.
Intended Use miRNA isolation for microarray analysis, Northern blotting, RT-PCR and miRNA library construction.
Principle / Technology Differential binding and silica-membrane purification of small RNA from lysed animal cell and tissue samples.
Detection Method Downstream miRNA and small-RNA analysis.
Sample Type Animal tissues and cultured animal cells.
Performance Range / Specifications Broad starting-input range; 50 reactions.
Reaction Conditions / Protocol Lyse and homogenize the sample, separate or reduce high-molecular-weight nucleic acids as directed, bind small RNA to the spin column, wash and elute.
Package Specifications 50 reactions.
Product Form Spin-column miRNA isolation kit.
Key Features High miRNA recovery; broad starting-input range; reduced large-RNA and genomic DNA carryover; simple workflow.
Compatibility Microarray, Northern blotting, RT-PCR and miRNA library-preparation workflows.
Application Notes / Precautions Maintain RNase-free conditions and select a sample input within the recommended range to preserve small-RNA recovery.

For research use only, not for clinical use.

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