- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Cationic Liposomal Transfection Reagent |
| Catalog No. | RESK-0220 |
| Description | This product is a versatile cationic liposomal transfection reagent suitable for the transfection of DNA, RNA, and oligonucleotides, delivering high transfection efficiency in most eukaryotic cells. Its unique formulation allows the reagent to be added directly to serum-containing medium: the presence of serum does not affect transfection efficiency, thereby reducing cell damage caused by serum removal. After transfection, there is no need to remove the nucleic acid–liposome complexes or replace the medium with fresh medium; if desired, the complexes can be removed 4–6 h after transfection. The reagent is supplied as a sterile liquid at a concentration of 10 mg/mL. For transfection in a 24-well plate, approximately 1.5 uL is used per well, so 1 mL supports about 660 transfections; for a 6-well plate, approximately 6 uL is used per well, so 1 mL supports about 160 transfections. |
| Intended Use | For research use in the transfection of DNA, RNA, and oligonucleotides into eukaryotic cells; suitable for both transient and stable transfection experiments. |
| Principle / Technology | Cationic liposome-mediated nucleic acid delivery. The liposomes form complexes with nucleic acids that can be added directly to serum-containing culture medium without loss of transfection efficiency. |
| Sample Type | Cultured eukaryotic (mammalian) cells. |
| Performance Range / Specifications | High efficiency: transfects most eukaryotic cells, with transfection efficiency above 90% in common cell lines; cells such as 293T, 293FT, and HeLa generally reach efficiencies above 85%. Low toxicity: transfected cells maintain good morphology and express high levels of the transfected gene product. Easy handling: liposome complexes can be added directly to serum-containing medium. Versatility: suitable for both transient and stable transfection. Concentration: 10 mg/mL. Approximately 1.5 uL per 24-well transfection (1 mL ≈ 660 transfections); approximately 6 uL per 6-well transfection (1 mL ≈ 160 transfections). |
| Components / Formulation | Cationic liposomal formulation, supplied as a sterile liquid at 10 mg/mL. |
| Storage Conditions | Store at 2–8 °C. Do not freeze; liposomes cannot withstand freezing, and 4 °C storage preserves optimal transfection performance. |
| Shelf Life | 1 year when stored at 2–8 °C. |
| Package Specifications | 100 uL 0.5 mL 1.0 mL 5 × 1 mL |
| Product Form | Sterile liquid |
| Concentration | 10 mg/mL |
| Shipping Conditions | Shipped on wet ice. |
| Compatibility | Suitable for DNA, RNA, and oligonucleotides. Compatible with serum-containing medium; serum does not affect transfection efficiency. Suitable for transient and stable transfection in most eukaryotic cells. |
| Application Notes / Precautions | No medium change to serum-free medium is required before transfection; the reagent works in serum-containing medium. No reaction termination is needed after transfection; liposome complexes remain stable for about 6 h. If the medium was not refreshed before transfection, replace the medium approximately 6 h after transfection to ensure adequate nutrients; if the medium was refreshed before transfection, change it as usual when nutrients are depleted. Single-plasmid transfection efficiencies are high in validated cell lines; for multi-plasmid co-transfection, efficiency depends on plasmid mixing ratios and reagent-to-plasmid ratios and should be verified empirically. Do not freeze the reagent; store at 2–8 °C for optimal performance. All cationic liposomal reagents show some cytotoxicity; this formulation has been optimized to markedly reduce toxicity while further improving transfection efficiency. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
