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| Product Name | Cationic Lipid Transfection Reagent, High Efficiency for Adherent Cells |
| Catalog No. | ATR-CTR-0001 |
| Description | A second-generation cationic lipid-based transfection reagent formulated for the highly efficient delivery of plasmid DNA into a wide variety of adherent mammalian cell lines with minimal cytotoxicity. |
| Intended Use | Transient and stable transfection of plasmid DNA into adherent mammalian cell lines for protein overexpression, reporter gene assays, shRNA expression, and gene function studies in research laboratories. |
| Principle / Technology | Cationic lipid molecules complex with negatively charged DNA phosphate backbone through electrostatic interactions to form lipoplexes; the net positive charge facilitates binding to the negatively charged cell surface glycocalyx, followed by endocytosis and endosomal escape. |
| Detection Method | Reporter gene expression (GFP, luciferase), antibiotic resistance colony formation for stable transfection, flow cytometric quantification of transfection efficiency |
| Sample Type | Adherent mammalian cell lines: HEK293, HeLa, COS-7, NIH/3T3, CHO-K1, A549, MCF-7, HCT116 |
| Performance Range / Specifications | Transfection efficiency 70–95% for HEK293 cells; 50–80% for HeLa and COS-7; cell viability >90% 24 hours post-transfection |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Dilute DNA in Opti-MEM or serum-free medium; dilute reagent separately; mix DNA and reagent at 1:3 ratio (w/w); incubate 5–20 min for complex formation; add complexes dropwise to cells in complete medium; assay 24–48 hours post-transfection. |
| Components / Formulation | Proprietary blend of cationic lipid, co-lipid (DOPE), and neutral lipid in ethanol-water vehicle, 0.2 µm filtered |
| Storage Conditions | 2–8°C; do not freeze |
| Shelf Life | 12 months |
| Package Specifications | 0.75 mL (sufficient for 125–250 transfections in 24-well format), 1.5 mL, 5 mL |
| Product Form | Liquid, sterile-filtered |
| Quality Control | Transfection efficiency and cytotoxicity testing with HEK293 and HeLa standard, sterility, endotoxin <0.5 EU/mL |
| Key Features | High efficiency; broad cell compatibility; serum-compatible protocol; ready to use; validated for common adherent cell lines; stable at 4°C |
For research use only, not for clinical use.
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