- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Carboxyl-Functionalized Magnetic Beads (3 µm) |
| Catalog No. | MAGBEA-0006 |
| Description | Three-micrometer carboxyl-functionalized superparamagnetic polymer beads with ultra-rapid magnetic separation characteristics, designed for applications requiring fast bead capture with minimal incubation times. The larger particle diameter accommodates higher total magnetite loading per bead, reducing magnetic separation time to under 3 seconds in standard racks. The surface carboxyl density is optimized for efficient covalent coupling of proteins and ligands via standard carbodiimide chemistry, making these beads particularly suitable for chemiluminescence immunoassay platforms and high-throughput bead-based ELISA systems. |
| Intended Use | Covalent immobilization of capture antibodies and antigens for automated chemiluminescence immunoassay (CLIA) platforms, bead-based multiplex immunoassay development, immunoprecipitation with fast magnetic capture, and industrial-scale affinity ligand coupling applications. |
| Principle / Technology | Uniform 3 µm superparamagnetic beads with a highly cross-linked polystyrene-divinylbenzene matrix, containing approximately 20-30% magnetite by weight distributed throughout the particle volume. The surface is densely functionalized with carboxylic acid groups introduced during polymerization and post-modification. Covalent protein coupling proceeds through EDC/sulfo-NHS activation of surface carboxyls, forming reactive NHS esters that react with primary amines on target proteins to create stable amide linkages with minimal ligand leaching under assay conditions. |
| Detection Method | Coulter counter and optical microscopy for particle size and monodispersity; SEM/TEM for surface morphology; conductometric titration for carboxyl density; BCA protein assay for coupling efficiency; zeta potential analysis for surface charge; automated CLIA testing for functional validation of coupled antibodies |
| Sample Type | Antibodies (monoclonal and polyclonal), recombinant antigens, streptavidin, enzymes, peptides, oligonucleotides with amino linkers, and any amine-containing biomolecule for covalent immobilization; optimized for coupling in MES activation buffer (pH 5.0-6.0) followed by PBS or borate coupling buffer (pH 7.4-9.0) |
| Performance Range / Specifications | Particle diameter: 3.0 ± 0.3 µm (CV <8%); carboxyl density: 0.3-1.0 mmol/g; IgG binding capacity: 50-120 µg/mg beads; non-specific binding: <3 µg BSA/mg; magnetite content: 20-30 wt%; magnetic separation time: <3 seconds (standard rack); sedimentation rate: fast settling—constant agitation required during processing; solid content: 10-50 mg/mL |
| Sensitivity / LOD | Antibody coupling detectable at input levels of 1 µg/mg; saturation at 80-120 µg/mg for most IgGs; coupled antibody retains >80% antigen-binding activity relative to free antibody; sub-picogram analyte detection achievable in optimized CLIA formats |
| Specificity | High specificity of covalent coupling chemistry for primary amines; minimal physical adsorption of proteins after blocking; no coupling to hydroxyl or carboxyl groups without activation; EDC-activated intermediates specific for amine nucleophiles; residual active groups efficiently quenched with ethanolamine; no detectable cross-reactivity between coupled antibodies and non-target analytes |
| Reaction Conditions / Protocol | Transfer desired volume of beads to reaction tube; wash twice with 50 mM MES pH 5.5; resuspend in activation buffer; add EDC (10-50 mM final) and sulfo-NHS (10-50 mM final); incubate 15-30 minutes at room temperature with rotation; wash twice with cold PBS pH 7.4; resuspend in coupling buffer; add protein at 50-150 µg/mg beads; incubate 2 hours room temperature or overnight at 4°C; wash; quench with 50 mM ethanolamine pH 8.0 for 30 minutes; wash extensively; resuspend in storage buffer; total coupling protocol: 3-5 hours; avoid prolonged stationary incubation to prevent bead settling |
| Components / Formulation | Carboxyl-functionalized superparamagnetic polymer beads, 3.0 µm, 10-50 mg/mL in deionized water or phosphate buffer with 0.02% sodium azide; compatible activation/coupling reagents (EDC, NHS/sulfo-NHS) not supplied |
| Storage Conditions | 2-8°C protected from light; do not freeze; store upright; vortex vigorously before each use until completely homogeneous; use aseptic technique for long-term storage after opening |
| Shelf Life | 24 months at 2-8°C; carboxyl density retention >85% at 24 months; accelerated stability at 37°C confirms equivalent 24-month shelf life; polymer integrity maintained without swelling or degradation |
| Package Specifications | 1 mL, 5 mL, 10 mL, 50 mL, 100 mL; concentrations of 10, 25, and 50 mg/mL; OEM and bulk packaging; trial sample packs available; supplied in polypropylene containers |
| Product Form | Dark brown to black thick suspension; rapid settling when stationary; homogeneous after 30-60 seconds of vortexing; uniform spherical particles by microscopy; smooth surface with consistent carboxyl density |
| Quality Control | Each lot QC: particle size distribution (Coulter), carboxyl density (titration), IgG coupling capacity, non-specific binding, magnetite content (TGA), endotoxin, bioburden, pH, preservative concentration; imaging by SEM for morphology; functional testing in model chemiluminescence immunoassay |
| Key Features | Ultra-fast <3 second magnetic separation; high magnetite loading for rapid capture; uniform 3 µm size for consistent bead handling; low non-specific binding polymer matrix; EDC/NHS coupling chemistry; validated for automated CLIA platforms; stable covalent linkage with minimal antibody leaching; suitable for industrial-scale production |
| Purity | >99% polymer-magnetite composite; endotoxin <0.1 EU/mg; heavy metals compliant with pharmaceutical limits; no extractable organic compounds above 10 ppm; sodium azide-free option available |
| Concentration | 10-50 mg/mL (labeled); verified gravimetrically per lot; particle count approximately 10^9-10^10 particles per mL at 10 mg/mL; exact particle count provided in lot certificate |
| Activity / Unit Definition | IgG coupling capacity: 50-120 µg/mg; carboxyl density: 0.3-1.0 mmol/g; coupling efficiency >85% under optimized conditions; antigen-binding capacity of coupled antibody >80% relative to free antibody |
| Molecular Weight | Not applicable for polymeric magnetic composite; each 3 µm bead estimated molecular weight ~10^11-10^12 g/mol; cross-linked structure prevents dissolution |
| Source / Origin | Entirely synthetic; polystyrene-divinylbenzene matrix formed by surfactant-free emulsion polymerization; magnetite nanoparticles synthesized by coprecipitation and encapsulated during polymerization; surface carboxylation by methacrylic acid copolymerization; all raw materials are synthetic chemicals; no biological source materials |
| pH Range / Optimal pH | Bead stability: pH 2.5-12.0; EDC activation optimum: pH 4.5-6.0; amine coupling optimum: pH 7.0-9.0; carboxyl group pKa ~4.5-5.0; bead integrity maintained within stated range; strong acids (pH <2) cause iron leaching; strong bases (pH >13) degrade polymer |
| Shipping Conditions | Ambient temperature (15-30°C); cold packs for extreme heat (>35°C); do not allow to freeze; standard courier acceptable; short transit times (<2 weeks) at ambient temperature do not affect bead performance |
| Expiration Date / Stability | 24-month shelf life verified by real-time monitoring; carboxyl density and coupling capacity maintained; polymer matrix shows no degradation; magnetic properties unchanged; preservative effective through expiry; opened containers: 12 months under sterile handling |
| Regulatory / Compliance | Research use and component manufacturing; suitable for IVD kit component after end-user validation; ISO 13485:2016 certified manufacturing; animal-origin-free certification; TSE/BSE statement available; CE marking as component upon request; quality agreement available for commercial supply |
| Compatibility | Compatible with all common EDC/NHS activation protocols; compatible with MES, PBS, borate, carbonate buffers; avoid Tris/glycine during activation (compete with target protein amines); coupled beads stable in common immunoassay buffers (PBS, Tris, HEPES) with surfactants (Tween-20, Triton X-100); compatible with automated bead handling on CLIA platforms |
| Recommended Buffer System | Storage buffer: deionized water or 10 mM sodium phosphate pH 7.4 with sodium azide preservative; activation: 50 mM MES pH 5.0-6.0; coupling: 50-100 mM PBS or borate pH 7.4-9.0; blocking: 50 mM ethanolamine or Tris pH 8.0; final storage: PBS pH 7.4 with 0.02% NaN3, 0.1% BSA, optional 5% glycerol |
| Application Notes / Precautions | Work quickly after bead vortexing due to rapid settling; use tube rotator or shaker during incubation steps; freshly prepare EDC and NHS solutions (hydrolysis half-life 2-4 hours in aqueous buffer); protein must be free of amine-containing buffer components; do not exceed protein loading capacity—excess may cause multilayer formation and increased non-specific binding; for sensitive antibodies, reduce EDC concentration to 1-5 mM; coupled bead aggregation can be minimized by brief sonication (<10 seconds, low power) |
| Batch-to-Batch Consistency | Particle size CV <8% inter-lot; carboxyl density CV <10%; IgG binding capacity CV <12%; magnetite content CV <10%; endotoxin <0.1 EU/mg across all production lots; SPC-controlled manufacturing with documented process capability |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
