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| Product Name | Calcium Phosphate Cell Transfection Kit |
| Catalog No. | RERE-0310 |
| Description | Kit implementing the classical calcium phosphate DNA coprecipitation method, suited to transfection of most adherent cell lines. On well-optimized conditions HEK293 cells, among the best matched targets for this chemistry, can reach transfection efficiencies above 85 percent, with typical runs landing around 40 to 50 percent. The method is economical and reagent-gentle, making it a practical choice for routine plasmid delivery at a 200-test scale. Store at -20 °C; shelf life is 6 months; aseptic handling is required. |
| Intended Use | DNA transfection of adherent cell lines by calcium phosphate coprecipitation |
| Principle / Technology | Calcium phosphate-DNA precipitate is taken up by cells through endocytosis and releases DNA for expression |
| Specificity | 200 T; suited to most adherent cells; HEK293 efficiency up to 85 percent when optimized, typically 40-50 percent; 6 month shelf life |
| Reaction Conditions / Protocol | Mix plasmid DNA with the calcium and buffer components to form the precipitate, add to the culture and replace the medium after incubation |
| Storage Conditions | -20 °C |
| Package Specifications | 200 T |
| Key Features | Classical chemistry with high HEK293 performance; economical test scale; 6 month shelf life |
For research use only, not for clinical use.
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