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| Product Name | Calcium Phosphate Cell Transfection Kit |
| Catalog No. | RESK-0272 |
| Description | DNA transfection by the calcium phosphate method is based on the formation of a calcium phosphate-DNA coprecipitate: calcium phosphate promotes the association of DNA with the cell membrane, after which exogenous DNA is delivered into target cells via endocytosis. This method has been used for the transfection of a wide variety of cell lines. This calcium phosphate cell transfection kit is specially optimized on the basis of the traditional calcium phosphate method. It not only greatly improves transfection efficiency but also reduces cytotoxicity. It is particularly suitable for the transfection of HEK293 or 293T cells and also delivers good transfection results in most other common cell lines, such as HeLa and CHO. The kit is suitable for both transient transfection of cells and the selection of stably transfected cell lines. The product is supplied sterile and is ready to use. |
| Intended Use | Transfection of exogenous DNA into mammalian cells; suitable for transient transfection and stable cell line selection. Especially recommended for HEK293 and 293T cells; also effective in HeLa, CHO, and most other common cell lines. Recommended for DNA transfection (not recommended for RNA transfection). For research use only. |
| Principle / Technology | Calcium phosphate forms a coprecipitate with DNA, which promotes binding of DNA to the cell membrane; the precipitate is then internalized by cells through endocytosis, delivering exogenous DNA into the target cells. |
| Sample Type | Adherent and suspension mammalian cell cultures (e.g., HEK293, 293T, HeLa, CHO). |
| Performance Range / Specifications | Stable performance: can be stored at 2-8 °C with a shelf life of 2 years. High transfection efficiency: positive rate up to 95% in 293T cells. Low cytotoxicity: essentially non-toxic to cells and does not affect normal cell growth after transfection. Application example: GFP plasmid transfection of 293T cells in a 6-well plate showed a transfection efficiency of more than 95% when observed under an inverted fluorescence microscope 48 h post-transfection. |
| Sensitivity / LOD | Transfection efficiency (GFP-positive rate) up to 95% in 293T cells. |
| Reaction Conditions / Protocol | Follow the recommended reaction system in the product manual: first add DNA slowly into the CaCl2 solution and mix thoroughly, then add the mixture slowly into the HBS buffer to form the calcium phosphate-DNA coprecipitate, which is subsequently applied to the cells. Transfection results are typically evaluated 48 h post-transfection. |
| Components / Formulation | CaCl2 solution HBS (HEPES-buffered saline) Sterile, ready-to-use formulation. |
| Storage Conditions | Store at 2-8 °C. |
| Shelf Life | 2 years when stored at 2-8 °C. |
| Package Specifications | 200 T |
| Product Form | Sterile liquid solutions, ready to use. |
| Quality Control | Sterile packaging; transfection performance validated in 293T cells (GFP-positive rate up to 95%). |
| Compatibility | Suitable for most common mammalian cell lines, especially HEK293 and 293T; also effective in HeLa and CHO cells. Applicable to both transient transfection and stable transfectant selection. Recommended for DNA transfection; not recommended for RNA transfection. |
| Recommended Buffer System | HBS (HEPES-buffered saline) with CaCl2 solution, supplied with the kit. |
| Application Notes / Precautions | Not recommended for RNA transfection; use for DNA transfection. Add reagents in the recommended order: DNA into CaCl2 solution first, then the mixture into HBS, adding slowly with thorough mixing. |
For research use only, not for clinical use.
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