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| Product Name | Blue Tracking Universal SYBR qPCR Master Mix |
| Catalog No. | NATR-HMM-0190 |
| Description | A blue tracking universal SYBR qPCR master mix designed for visible pipetting, broad quantification range and reliable standard or fast amplification. |
| Intended Use | Relative gene-expression analysis; absolute quantification; routine dye-based real-time PCR. |
| Principle / Technology | A hot-start DNA polymerase, optimized buffer, specificity enhancer, intercalating dye and passive-reference system support efficient qPCR while the blue color provides visual pipetting guidance. |
| Detection Method | Intercalating-dye real-time PCR. |
| Sample Type | DNA or cDNA templates. |
| Performance Range / Specifications | 500 or 2,500 reactions; supports a broad quantitative range and both standard and fast qPCR programs. |
| Specificity | Hot-start amplification and the optimized buffer system reduce nonspecific products. |
| Reaction Conditions / Protocol | Add primers and template to the blue tracking master mix and run a compatible standard or fast real-time PCR program. |
| Components / Formulation | qPCR master mix containing hot-start DNA polymerase, intercalating dye, optimized buffer, specificity enhancer, passive-reference dye and blue tracking dye. |
| Storage Conditions | Store at −30 to −15°C protected from light. |
| Package Specifications | 500 reactions; 2,500 reactions. |
| Product Form | Blue tracking 2× SYBR qPCR master mix. |
| Key Features | Visible pipetting; broad quantification range; high specificity; standard and fast program compatibility; broad instrument compatibility. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | Stable for up to 18 months at −20°C, 7 days at 37°C and 50 freeze–thaw cycles under the evaluated conditions. After thawing, store at 2–8°C for up to 1 month. A complete reaction mixture remains stable for up to 6 hours at 2–8°C. |
| Compatibility | Compatible with common real-time PCR instruments through an optimized passive-reference system. |
For research use only, not for clinical use.
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