| Product Name | Blood Ammonia Content Assay Kit (Visible Spectrophotometers) |
| Catalog No. | BBITK-HMM-0017 |
| Description | The main sources of blood ammonia are endogenous ammonia and exogenous ammonia. The content of blood ammonia remains stable in the blood and is mainly metabolized and detoxified in the liver. When liver function is severely impaired, ammonia cannot be detoxified and accumulates in the central nervous system, thereby leading to hepatic encephalopathy. |
| Testing Equipment | Visible Spectrophotometers |
| Matching | 1 mL glass cuvette (d=10 mm) |
| Number of Testable Samples | 48 Samples |
| Estimated Measurement Time | 2 h (48 Samples) |
| Storage | Store at 4°C |
| Self-contained Reagents | / |
| Detection Principle | After protein precipitation from serum (plasma) samples by protein precipitant, blood ammonia is measured by direct phenol-hypochlorite chromatography, the blue indophenol generated is directly proportional to the concentration of ammonia, and the product has a characteristic absorption peak at 630 nm, which can be used to quantitatively detect the content of blood ammonia by the change of absorbance value. |
| Detection Methods | Indophenol Blue Method |
| Detection Wavelength | 630 nm |
| Signal Response | Incremental |
| Standard | Nitrogen |
| Note | If A exceeds the linear range of the standard absorbance value: if it exceeds the highest value, it is recommended to dilute the sample to be tested or the supernatant appropriately before measurement; if it is lower than the lowest value, it can be measured by increasing the sample volume appropriately, and the calculation can be modified accordingly; the equipment and blood sampling device should be free of ammonia, and the measurement should be carried out immediately after the collection of blood, and the hemolysed sample should not be used. |
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