| Product Name | Blood Zinc Content Assay Kit (Microplate Reader) |
| Catalog No. | BBITK-HMM-0014 |
| Description | Zinc, as one of the important trace elements, participates in the composition of various coenzymes and plays a significant role in insulin, porphyrin metabolism, intellectual development and maintaining the immune function of the body. Both excessively high and low blood zinc levels can affect the normal physiological functions of the body. |
| Testing Equipment | Microplate Reader |
| Matching | 96-well plate |
| Number of Testable Samples | 96 Samples |
| Estimated Measurement Time | 3 h (96 Samples) |
| Storage | Store at 4°C |
| Self-contained Reagents | / |
| Detection Principle | In pH=8.5-9.5 solution, Zn2+ can react with zinc reagent to form coordination compounds, and the product has a characteristic absorption peak at 620 nm, which can be quantitatively detected by the change of absorbance value. |
| Detection Methods | Complexation Colorimetry Method |
| Detection Wavelength | 620 nm |
| Signal Response | Incremental |
| Standard | Zinc |
| Note | To ensure accurate results and avoid loss of reagents, please read the instruction manual carefully before the measurement, make sure the reagents are adequately stored and prepared, and the operation steps are clear, and be sure to take 2-3 samples with expected differences for pre-calibration. |
Zinc, an essential trace element in biological systems, is a cornerstone of cellular function and metabolic regulation—playing irreplaceable roles in over 300 enzymatic reactions, gene expression modulation, and maintenance of structural integrity of proteins. In research contexts, understanding blood zinc levels is critical for unraveling its involvement in diverse biological processes: for example, in developmental biology, zinc deficiency has been linked to impaired cell proliferation and differentiation in embryonic models; in immunology research, zinc regulates the activation of T lymphocytes and cytokine production, making it a key variable in studies on immune response mechanisms; and in metabolic science, zinc’s interaction with insulin signaling pathways is a focal point for investigating diabetes and metabolic syndrome in cellular and animal models.
Abnormal blood zinc concentrations—whether deficient or excessive—can drastically alter experimental outcomes, making accurate quantification a non-negotiable step in many life science studies. For instance, in nutritional physiology research, inconsistent zinc levels in animal models can skew data on growth rates or nutrient absorption efficiency; in toxicology studies, excessive zinc exposure may induce oxidative stress in blood cells, interfering with assessments of other toxicant effects. As such, reliable blood zinc detection tools are foundational to ensuring the reproducibility and validity of research findings across disciplines like molecular biology, pharmacology, and environmental health.
Traditional blood zinc detection methods, such as atomic absorption spectrometry (AAS) and inductively coupled plasma mass spectrometry (ICP-MS), while highly sensitive, present significant barriers for many research laboratories: they require expensive, specialized equipment; demand rigorous sample pretreatment (e.g., acid digestion, matrix separation); and often necessitate trained personnel to operate and maintain. These limitations restrict their accessibility, particularly for small to mid-sized research teams, academic labs with limited budgets, or projects requiring high-throughput sample processing.
The development of microplate reader-based blood zinc assay kits addresses these challenges head-on. By leveraging the widespread availability of microplate readers in most research facilities, this kit eliminates the need for costly specialized equipment, streamlines sample handling procedures, and enables batch processing—aligning with the growing demand for efficient, accessible analytical tools in modern research. Furthermore, as the global life sciences research market continues to expand (driven by investments in personalized medicine, environmental toxicology, and animal model studies), the need for reliable, user-friendly trace element detection kits like this one has become increasingly pronounced, making it a valuable asset for researchers worldwide.
Research-Tailored Specificity: Optimized to detect Zn²⁺ in blood samples with minimal interference from other common biological matrices (e.g., proteins, lipids) and co-existing trace elements (e.g., copper, iron) that often confound detection. The pH 8.5-9.5 reaction environment ensures stable coordination complex formation between Zn²⁺ and the zinc reagent, guaranteeing specificity critical for research data accuracy.
High-Throughput Compatibility: Designed for use with standard 96-well plates, enabling simultaneous processing of up to 96 blood samples in a single run. This feature is ideal for research projects involving large sample cohorts (e.g., epidemiological studies on environmental zinc exposure, preclinical drug trials assessing metabolic impacts) where efficient data collection is essential.
Streamlined Protocol for Research Efficiency: Requires no complex sample pretreatment (e.g., no acid digestion or extensive centrifugation) beyond basic blood sample preparation (e.g., serum/plasma separation). The simplified workflow reduces hands-on time, minimizes the risk of sample loss or contamination, and allows researchers to focus on core experimental objectives.
Stable Storage for Laboratory Convenience: Formulated for storage at 4°C—a standard refrigeration condition in most research labs—eliminating the need for ultra-low temperature (-20°C or -80°C) storage. This not only reduces logistics and storage costs but also ensures reagents remain stable for extended periods, supporting long-term research projects with intermittent testing needs.
Quantitative Calibration for Research Reproducibility: Includes a zinc standard to generate standard curves, enabling precise conversion of 620 nm absorbance values into absolute zinc concentration data. This quantitative capability is vital for research applications (e.g., comparing zinc levels across experimental groups, measuring dose-response effects) where numerical accuracy and data comparability are required.
Cost-Effective for Research Budgets: Eliminates the need for investment in expensive AAS or ICP-MS equipment, leveraging existing microplate readers that are standard in most research laboratories. This significantly reduces upfront costs, making high-quality blood zinc detection accessible to academic labs, early-stage research teams, and institutions with limited funding.
Flexible for Diverse Research Sample Types: Validated for use with multiple blood-derived samples common in research (e.g., whole blood, serum, plasma from rodents, non-human primates, or human cell culture models). This flexibility eliminates the need to purchase separate kits for different sample types, simplifying inventory management and reducing research expenses.
Consistent Results for Reproducible Research: The complexation colorimetry method delivers consistent absorbance readings across multiple runs and operators, a key requirement for research studies where data reproducibility is essential for peer review and publication. Pre-calibration recommendations (2-3 samples with expected differences) further enhance result reliability.
Time-Efficient for Fast-Paced Research: Completes the entire detection process (from sample loading to data acquisition) in approximately 3 hours for 96 samples—far faster than traditional AAS/ICP-MS methods, which often require hours of sample pretreatment and instrument setup. This speed accelerates research timelines, enabling quicker data analysis and experimental iteration.
Safe for Laboratory Environments: Reagents are non-toxic, non-corrosive, and free of hazardous substances (e.g., no strong acids, radioactive markers, or toxic heavy metals). This minimizes safety risks for researchers, simplifies waste disposal in compliance with laboratory safety protocols, and eliminates the need for specialized protective equipment during handling.
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2025 Alta DiagenoTech. All rights reserved.
