| Product Name | Blood Ammonia Content Assay Kit (Microplate Reader) |
| Catalog No. | BBITK-HMM-0018 |
| Description | The main sources of blood ammonia are endogenous ammonia and exogenous ammonia. The content of blood ammonia remains stable in the blood and is mainly metabolized and detoxified in the liver. When liver function is severely impaired, ammonia cannot be detoxified and accumulates in the central nervous system, thereby leading to hepatic encephalopathy. |
| Testing Equipment | Microplate Reader |
| Matching | 96-well plate |
| Number of Testable Samples | 96 Samples |
| Estimated Measurement Time | 3 h (96 Samples) |
| Storage | Store at 4°C |
| Self-contained Reagents | / |
| Detection Principle | After the protein is precipitated from serum (plasma) samples by protein precipitant, blood ammonia can be determined by phenol-hypochlorite direct chromatography, the blue indophenol generated is proportional to the concentration of ammonia, and the product has a characteristic absorption peak at 630 nm, which can be quantitatively detected by the change of absorbance value. |
| Detection Methods | Indophenol Blue Method |
| Detection Wavelength | 630 nm |
| Signal Response | Incremental |
| Standard | Nitrogen |
| Note | If A exceeds the linear range of the standard absorbance value: if it exceeds the highest value, it is recommended to dilute the sample to be tested or the supernatant appropriately before measurement; if it is lower than the lowest value, it can be measured by increasing the sample volume appropriately, and the calculation can be modified accordingly; the equipment and blood sampling device should be free of ammonia, and the measurement should be carried out immediately after the collection of blood, and the hemolysed sample should not be used. |
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