| Product Name | Blood Ammonia Content Assay Kit (Microplate Reader) |
| Catalog No. | BBITK-HMM-0018 |
| Description | The main sources of blood ammonia are endogenous ammonia and exogenous ammonia. The content of blood ammonia remains stable in the blood and is mainly metabolized and detoxified in the liver. When liver function is severely impaired, ammonia cannot be detoxified and accumulates in the central nervous system, thereby leading to hepatic encephalopathy. |
| Testing Equipment | Microplate Reader |
| Matching | 96-well plate |
| Number of Testable Samples | 96 Samples |
| Estimated Measurement Time | 3 h (96 Samples) |
| Storage | Store at 4°C |
| Self-contained Reagents | / |
| Detection Principle | After the protein is precipitated from serum (plasma) samples by protein precipitant, blood ammonia can be determined by phenol-hypochlorite direct chromatography, the blue indophenol generated is proportional to the concentration of ammonia, and the product has a characteristic absorption peak at 630 nm, which can be quantitatively detected by the change of absorbance value. |
| Detection Methods | Indophenol Blue Method |
| Detection Wavelength | 630 nm |
| Signal Response | Incremental |
| Standard | Nitrogen |
| Note | If A exceeds the linear range of the standard absorbance value: if it exceeds the highest value, it is recommended to dilute the sample to be tested or the supernatant appropriately before measurement; if it is lower than the lowest value, it can be measured by increasing the sample volume appropriately, and the calculation can be modified accordingly; the equipment and blood sampling device should be free of ammonia, and the measurement should be carried out immediately after the collection of blood, and the hemolysed sample should not be used. |
Blood ammonia, a pivotal metabolite in the nitrogen metabolism pathway of organisms, is an essential research target in fields such as hepatology, neurobiology, and metabolic biology. Its concentration in biological samples is tightly regulated by metabolic processes—primarily detoxified through the urea cycle in the liver of mammals. In research scenarios, studying blood ammonia levels provides critical insights into understanding how various biological and environmental factors disrupt metabolic homeostasis, particularly in the context of liver-related and neuro-metabolic research models.
In laboratory settings, blood ammonia analysis is widely applied across multiple research directions:
Liver Metabolism Research: When constructing animal models of liver injury (e.g., carbon tetrachloride-induced cirrhosis, acetaminophen-induced acute liver failure) or cell models of hepatocyte damage, monitoring blood ammonia changes helps evaluate the degree of liver function impairment. Research data shows that in rodent liver injury models, a significant increase in blood ammonia concentration (often exceeding 120 μmol/L in mice) is closely associated with the decline in key urea cycle enzyme activities (such as carbamoyl phosphate synthetase I).
Neuro-Metabolic Mechanism Studies: Elevated blood ammonia can cross the blood-brain barrier, leading to changes in cerebral energy metabolism and neurotransmitter balance. In studies on hepatic encephalopathy (HE) mechanisms, researchers use blood ammonia assay kits to correlate ammonia levels with neuronal apoptosis, astrocyte swelling, and changes in glutamate/GABA neurotransmitter systems—providing a basis for exploring potential therapeutic targets for HE.
Inherited Metabolic Disease Research: In studies on animal models of urea cycle enzyme deficiencies (e.g., ornithine transcarbamylase deficiency), blood ammonia detection is a core indicator for confirming the success of model construction and evaluating the efficacy of gene therapy or drug intervention.
Traditional research-grade blood ammonia detection methods, such as gas chromatography-mass spectrometry (GC-MS) and ion-selective electrode methods, have drawbacks that limit their application in high-throughput research. GC-MS requires complex sample pretreatment and expensive equipment, while ion-selective electrodes have poor stability in batch testing and are prone to interference from sample matrices. Our Blood Ammonia Content Assay Kit (Microplate Reader) is developed for research purposes, addressing these limitations with its simple operation, high throughput, and good compatibility with common laboratory equipment—meeting the needs of in vitro research on animal serum, plasma, or cell culture supernatant samples.
Optimized for Research Sample Types: Specifically designed to handle common research samples, including animal serum, plasma (EDTA or heparin anticoagulated), and cell culture supernatant. The kit includes a dedicated protein precipitant that effectively removes interfering proteins in animal samples (e.g., albumin, globulin in rodent plasma) without affecting ammonia detection—ensuring accuracy in complex biological matrices.
Flexible Detection Volume: Supports adjustable sample volumes (20–100 μL) to accommodate different research needs. For samples with low ammonia concentrations (e.g., normal mouse plasma, ~30–60 μmol/L), increasing the sample volume to 100 μL enhances detection sensitivity; for high-concentration samples (e.g., ammonia-loaded cell supernatant), reducing the volume to 20 μL avoids exceeding the linear range—improving the adaptability of the kit to diverse research scenarios.
Compatible with Standard Laboratory Equipment: Works seamlessly with all mainstream microplate readers that support 630 nm wavelength detection. No specialized or custom equipment is required, allowing research teams to integrate the kit into existing experimental workflows without additional equipment investment.
Stable Reagent Formulation for Batch Research: All reagents are provided in ready-to-use or easy-to-reconstitute forms, with a 12-month shelf life when stored at 4°C. After reconstitution, the chromogenic reagent remains stable for 7 days under 4°C storage—ensuring consistent results across long-term batch experiments (e.g., a 2-week rodent model study with multiple time-point samplings).
High Throughput for Large-Scale Research Projects: Enables simultaneous detection of 96 samples within 3 hours, significantly improving experimental efficiency compared to traditional methods (e.g., GC-MS takes 1–2 hours per sample). This is particularly beneficial for high-throughput studies, such as drug screening for liver protection (evaluating ammonia levels in 50+ treatment groups) or genetic knockout mouse phenotyping (analyzing samples from multiple litters).
Cost-Effective for Routine Research Use: The 100T/96S packaging design minimizes reagent waste, with a per-sample test cost approximately 25% lower than imported research-grade ammonia assay kits. For laboratories conducting regular blood ammonia testing (e.g., 20–30 samples per week), this kit reduces long-term research costs without compromising data quality.
Minimal Technical Barrier for Research Teams: The protocol includes step-by-step instructions with clear time and temperature parameters (e.g., 37°C incubation for 15 minutes, room temperature chromogenic reaction for 20 minutes). Novice researchers or laboratory technicians can master the operation within 1–2 training sessions, reducing human error in batch experiments.
Good Anti-Interference Performance in Research Scenarios: Resistant to common interfering substances in research samples, such as bilirubin (up to 400 μmol/L, typical in jaundiced rodent models), triglycerides (up to 6.0 mmol/L), and common drugs (e.g., lactulose, a frequently used reagent in HE research). This ensures accurate results even when analyzing samples from models with complex physiological states.
Compliant with Research Data Standards: The detection principle (indophenol blue method) aligns with widely accepted research protocols in the field of ammonia analysis. Test results show good correlation (R² > 0.99) with reference methods (e.g., GC-MS) in inter-laboratory validation—ensuring that data generated using this kit is comparable to published research and suitable for inclusion in scientific papers or research reports.
For research use only, not for clinical use.
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