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| Product Name | Plasmid Extraction Kit (20 rxns) |
| Catalog No. | SD-HMM-0013 |
| Description | The Plasmid Kit uses an improved SDS base lysis method and dual magnetic bead extraction, avoiding high-speed centrifugation or vacuum filtration, which can realize rapid removal of impurities and specific binding of plasmid DNA, respectively. This kit is suitable for extracting plasmid from 1-50mL overnight culture of E.coli, and the extracted plasmid can be used for molecular biology experiments, such as sequencing, in vitro transcription and translation, restriction endonuclease digestion, bacterial transformation, etc. The plasmid can be extracted by using the SDS base lysis method, avoiding high-speed centrifugation or vacuum filtration. |
| Product Introduction | Plasmid DNA extraction is a routine yet critical procedure in molecular biology for cloning, sequencing, transfection, and other downstream applications. This kit utilizes an optimized alkaline lysis method combined with a spin-column purification technology to deliver high-quality, ready-to-use plasmid DNA from bacterial cultures efficiently. |
| Product Application | • Molecular cloning and subcloning • Preparation of DNA for sequencing • Generation of transfection-grade DNA • Restriction enzyme digestion and other enzymatic reactions |
| Product Advantages | • High-Purity Yield: The silica-membrane spin columns are designed to selectively bind plasmid DNA, effectively removing contaminants like endotoxins, proteins, and RNA, resulting in high A260/A280 ratios. • Rapid Protocol: The entire procedure, from bacterial pellet to eluted DNA, can be completed in under 30 minutes, offering a significant speed advantage over traditional phenol-chloroform methods. • Scalable Workflow: The kit's protocol is easily scalable for different culture volumes (from mini- to midi-preps), providing flexibility to match the yield requirements of your specific experiment. • Ready-to-Use DNA: The purified plasmid DNA is of high quality and suitable for sensitive downstream applications, including direct use in transfection, sequencing, and enzymatic digests without the need for further purification. |
For research use only, not for clinical use.
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