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| Product Name | Mag COOH-S500 nm |
| Catalog No. | SM-HMM-0004 |
| Description | Silicon dioxide series magnetic microspheres are specially designed for nucleic acid extraction and purification. The surface is silica material, modified with a large number of OH or COOH groups, and can bind to nucleic acids in solution through hydrophobic, hydrogen bonding and electrostatic interaction under high salt and low pH conditions, without binding with other impurities (such as proteins), and quickly separate nucleic acids from biological samples. The operation is safe and simple, which is conducive to the automation and high throughput extraction of nucleic acid. |
| Features | Super paramagnetic and high magnetic responsiveness, saving operation time and increasing sample recovery efficiency. Good suspension performance, dispersion and resuspension, which facilitates efficient binding and recovery of nucleic acids. Good physical and chemical stability to ensure reproducibility. |
| Average Particle Size | 500 nm |
| Magnetic Core | Fe3O4 |
| Shell | SiO2 |
| Magnetism Type | Superparamagnetic |
| Saturation Magnetization Strength | 50 emu/g |
| Preservation Fluid | 50 mM NaCl (sterile water) |
| Concentration | 10 mg/mL |
| Applications | Suitable for viral DNA/RNA extraction, cfDNA extraction, etc. |
Nucleic acid extraction and purification are foundational steps in modern life science research, underpinning a wide range of applications from genetic sequencing and gene expression analysis to pathogen detection and molecular cloning. As research demands grow more rigorous—with requirements for higher purity, faster processing, and greater reproducibility—traditional extraction methods (such as phenol-chloroform extraction) have gradually shown limitations, including complex operation procedures, long processing times, potential exposure to toxic reagents, and difficulty in scaling for high-throughput experiments.
To address these challenges, magnetic microsphere-based nucleic acid extraction technology has emerged as a game-changing solution in the scientific community. These microspheres leverage specific surface modifications and magnetic responsiveness to enable efficient, safe, and scalable nucleic acid separation. Among various magnetic microsphere products, silica-coated magnetic microspheres have gained widespread recognition due to their unique surface chemistry and compatibility with diverse biological samples.
Mag COOH-S500 nm is a high-performance silica-based magnetic microsphere tailored explicitly for research-grade nucleic acid extraction. Its design integrates advanced material science and molecular binding principles: the Fe₃O₄ magnetic core provides strong superparamagnetic properties, ensuring rapid separation under an external magnetic field, while the SiO₂ shell is functionalized with abundant carboxyl (COOH) groups. These groups facilitate specific and stable binding to nucleic acids (DNA/RNA) under high-salt and low-pH conditions—through a combination of hydrophobic interactions, hydrogen bonding, and electrostatic forces—while minimizing non-specific binding to proteins, lipids, and other impurities.
Superparamagnetic core with high magnetic responsiveness: Enables fast and efficient separation of microspheres from samples within seconds under an external magnetic field, significantly reducing operation time compared to conventional methods.
Excellent suspension and dispersion properties: Maintains uniform distribution in aqueous solutions without easy aggregation, ensuring full contact with nucleic acids in samples for maximized binding efficiency.
Easy resuspension capability: Can be quickly and completely resuspended with gentle mixing after magnetic separation, avoiding loss of microspheres and ensuring consistent recovery rates.
Robust physical and chemical stability: Resists degradation under a wide range of experimental conditions (e.g., pH 3–9, common buffer systems, and moderate temperature variations), guaranteeing reproducible results across multiple experiments.
Precisely controlled particle size (500 nm average): Optimized for balanced binding capacity and separation efficiency—large enough to carry abundant nucleic acids, yet small enough for efficient dispersion in sample solutions.
Sterile preservation system: Supplied in 50 mM NaCl sterile water, ensuring no contamination of research samples and eliminating the need for additional sterilization steps before use.
High concentration (10 mg/mL): Provides sufficient material for multiple experiments, reducing the frequency of product replacement and supporting high-throughput research needs.
High nucleic acid purity: The COOH-functionalized silica surface ensures specific binding to nucleic acids, effectively excluding proteins, carbohydrates, and other impurities, resulting in high-purity nucleic acid eluates suitable for downstream applications (e.g., PCR, qPCR, sequencing).
Superior recovery efficiency: Optimized surface group density and magnetic responsiveness minimize nucleic acid loss during binding, washing, and elution steps, especially for low-abundance targets like cfDNA and viral nucleic acids.
Simplified workflow: Eliminates the need for centrifugation or filtration, reducing operational complexity and the risk of cross-contamination between samples—ideal for automated and high-throughput research platforms.
Wide sample compatibility: Performs reliably with various research samples, including cell cultures, tissue homogenates, blood, plasma, saliva, and viral supernatants, adapting to diverse research needs.
Cost-effective for research use: High concentration and efficient performance mean less product is required per experiment, lowering the overall research cost without compromising quality.
Consistent batch-to-batch performance: Strict quality control during production ensures minimal variation between batches, providing researchers with reliable and reproducible results for long-term projects.
For research use only, not for clinical use.
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