Human CD3/CD28 T Cell Activation Beads

Cat.No: SM-HMM-0039 Datasheet

Specification Quantities

1×10^8 cells:

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Product Details Product Overview Related Products

Product Name	Human CD3/CD28 T Cell Activation Beads
Catalog No.	SM-HMM-0039
Description	Human CD3/CD28 T Cell Activation Magnetic Beads allow for simple and rapid activation and expansion of T cells without the need for feeder cells (antigen-presenting cells) or antigens. The beads are inert, super-paramagnetic beads 5 µm in diameter, similar in size to antigen-presenting cells, and are covalently coupled with both anti-CD3 and anti-CD28 antibodies. Recombinant human IL-2 can be used to stimulate T cell population expansion during cell culture. After activation or expansion, the magnetic beads can be easily removed by a magnetic rack.
Storage	Store at 2-8°C, do not freeze.
Concentration	1 x 10^8 beads/mL
Applications	This kit is intended for use with peripheral blood mononuclear cells (PBMC) or T cell subsets: including CD3+ T cells, CD4+ T cells, or CD8+ T cells.

Product Introduction	These magnetic beads are coated with antibodies against CD3 and CD28, providing a potent and specific stimulus to activate and expand human T cells. CD3 engagement mimics TCR signaling, while CD28 provides a critical co-stimulatory signal. This method is superior to soluble antibodies for generating robust, sustained T cell activation and proliferation, essential for immunotherapy research, CAR-T development, and fundamental immunology studies.
Product Application	• In vitro T cell activation and expansion for research • CAR-T cell and adoptive cell therapy development • Studies of T cell signaling, differentiation, and exhaustion • Antigen-specific T cell culture
Product Advantages	• Dual-Signal Activation: Provides both Signal 1 (via CD3) and Signal 2 (via CD28) in a physiological ratio on the bead surface, mimicking antigen-presenting cell interaction for optimal activation. • Magnetic Convenience: The beads are paramagnetic, allowing for easy removal from the cell culture after activation via a simple magnet, preventing ongoing stimulation if desired. • Consistent & Defined Stimulus: Offers a reproducible, defined, and controllable activation signal compared to variable antigen-presenting cells, leading to more consistent experimental results. • High Expansion Capacity: Promotes vigorous T cell proliferation, enabling the generation of large numbers of cells for downstream assays or therapeutic applications.

Alta DiagnoTech supports the complete workflow for T cell research and immunotherapy development. Our human CD3/CD28 T cell activation beads are the cornerstone for robust T cell stimulation. To prepare pure cell populations for activation, we offer human T cell isolation kits (negative or positive selection). Following activation, the functional output can be precisely quantified using our extensive range of human cytokine ELISA kits (e.g., IL-2, IFN-γ). For downstream therapeutic applications like CAR-T generation, our nucleofection kits or viral transduction reagents can be integrated into this workflow.

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For research use only, not for clinical use.