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| Product Name | Bacterial Genomic DNA Mini Extraction Kit |
| Catalog No. | DREK-0096 |
| Description | A silica-column kit for isolating high-quality genomic DNA from Gram-positive and Gram-negative bacteria. The workflow avoids phenol/chloroform and alcohol precipitation while removing RNA, protein, lipid and other inhibitory contaminants. |
| Intended Use | Bacterial genomic DNA preparation for restriction digestion, PCR, qPCR, Southern blotting, library construction and next-generation sequencing. |
| Principle / Technology | Bacterial lysis followed by selective silica-membrane binding, washing and elution. |
| Detection Method | Downstream genomic DNA analysis. |
| Sample Type | Gram-negative and Gram-positive bacterial cultures. |
| Performance Range / Specifications | Approximately 30-minute extraction; high yield, high purity and strong genomic DNA integrity; 100 reactions. |
| Specificity | Optimized lysis and purification conditions recover bacterial genomic DNA while reducing RNA, protein, lipid and other inhibitors. |
| Reaction Conditions / Protocol | Harvest bacteria, perform the applicable lysis treatment, establish binding conditions, load the lysate onto the spin column, wash and elute. |
| Components / Formulation | Bacterial lysis, binding, wash and elution reagents with silica spin columns and collection tubes. |
| Storage Conditions | Store at 15-25°C. |
| Package Specifications | 100 reactions. |
| Product Form | Bacterial genomic DNA spin-column kit. |
| Quality Control | DNA yield, purity, integrity and PCR compatibility are verified with representative Gram-positive and Gram-negative bacteria. |
| Key Features | Approximately 30-minute workflow; broad bacterial compatibility; phenol-free procedure; high-integrity genomic DNA. |
| Shipping Conditions | Ship at room temperature. |
| Compatibility | Restriction digestion, PCR, qPCR, Southern blotting and sequencing workflows. |
| Application Notes / Precautions | Use an appropriate pretreatment for the bacterial cell wall type and avoid exceeding the recommended cell input. |
| Batch-to-Batch Consistency | Bacterial DNA yield and purity are controlled across production batches. |
For research use only, not for clinical use.
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