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| Product Name | Bacterial Genomic DNA Extraction Kit (100 rxns) |
| Catalog No. | SD-HMM-0006 |
| Description | The Bacterial Genomic DNA Kit can extract genomic DNA from bacterial samples based on magnetic beads method. Using magnetic beads after lysis of Gram positive or negative bacteria, its genomic DNA will be enriched and extracted with high efficiency and purity. The extracted sample can be directly used in enzyme digestion, PCR, next generation sequencing, gene chip, Southern hybridization and other experiments. |
| Features | Fast operation: The experiment duration is 40 mins. No contamination: No RNA and protein contamination. Wide range: Applicable to all bacteria. No poison: No toxic organic solvents such as phenol and chloroform. |
| Storage | At 2-35°C |
| Shelf Life | 2 years |
| Components | Amount |
| Beaver Beads | 2 mL |
| Buffer AL | 30 mL |
| Buffer LB | 25 mL |
| Buffer W1 | 60 mL |
| Buffer EB | 20 mL |
| Proteinase K | 2 mL |
| Lysozyme | 360mg |
Genomic DNA extraction is a fundamental step in bacterial research, serving as the foundation for downstream applications such as gene cloning, sequence analysis, and functional genomics. With the rapid development of molecular biology techniques, the demand for efficient, reliable, and safe DNA extraction methods has grown significantly across academic research institutions, biotechnology companies, and pharmaceutical R&D laboratories.
Bacteria, including both Gram-positive and Gram-negative strains, have unique cell wall structures and cellular components that can interfere with DNA isolation. Traditional extraction methods often involve complex steps, toxic reagents, or lengthy processing times, leading to low yields, degraded DNA, or contamination—issues that hinder subsequent experimental accuracy and efficiency.
Magnetic bead-based extraction technology has emerged as a game-changer in nucleic acid purification. By leveraging the specific binding between magnetic beads and DNA under optimized buffer conditions, this method simplifies the extraction process while ensuring high purity and yield. Our Bacterial Genomic DNA Extraction Kit adopts this advanced magnetic bead technology, addressing the limitations of traditional methods and meeting the rigorous requirements of modern bacterial research.
This product is designed exclusively for research purposes and is not intended for clinical diagnosis, treatment, or any medical applications. It provides researchers with a consistent and user-friendly tool to obtain high-quality genomic DNA, supporting a wide range of experimental objectives from basic bacterial characterization to advanced molecular genetics studies.
Fast operation workflow that completes the entire extraction process in just 40 minutes, significantly reducing experimental time compared to conventional methods (which typically take 1-2 hours or longer).
High-purity DNA output with no RNA or protein contamination, ensuring compatibility with sensitive downstream applications such as PCR, qPCR, and next-generation sequencing (NGS).
Broad applicability covering all bacterial types, including Gram-positive bacteria (e.g., Staphylococcus, Bacillus) and Gram-negative bacteria (e.g., Escherichia coli, Salmonella), eliminating the need for specialized kits for different strains.
Non-toxic and safe formulation, free of harmful organic solvents like phenol and chloroform, reducing health risks to operators and minimizing environmental impact.
Stable performance with consistent results across multiple experiments, thanks to rigorously optimized buffers and magnetic bead materials that ensure reproducibility.
User-friendly protocol with clear, step-by-step instructions, suitable for both experienced researchers and laboratory beginners, reducing the learning curve and operational errors.
Flexible sample compatibility, capable of handling various bacterial sample types such as liquid cultures, colony samples, and environmental isolates (e.g., from soil, water, or food).
High extraction efficiency that recovers 80-95% of genomic DNA from bacterial samples (depending on strain and sample volume), providing sufficient yield for multiple downstream experiments.
DNA integrity preservation, producing high-molecular-weight genomic DNA with minimal shearing, which is critical for applications like Southern hybridization and gene chip analysis.
Time and cost savings, as the simplified workflow reduces labor input and the kit’s all-in-one composition eliminates the need for additional reagents or equipment.
Robust performance under different experimental conditions, maintaining stability and reliability even with variations in sample concentration or handling.
Long shelf life of 2 years when stored at 2-35°C, offering convenient storage and inventory management without the need for refrigeration or freezing (except for specific buffer components as noted in the manual).
Research-focused design aligned with the needs of academic and industrial research, ensuring that the product meets the strict quality standards required for scientific experiments.
For research use only, not for clinical use.
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