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| Product Name | Bacterial and Fungal Total RNA Extraction Kit |
| Catalog No. | DREK-0105 |
| Description | A two-reagent extraction system optimized for prokaryotic and fungal samples. A pretreatment reagent suppresses endogenous RNases and denatures proteins before strong phenol/guanidinium lysis and RNA recovery. |
| Intended Use | Total RNA isolation from bacteria and compatible fungi for qPCR, RT-PCR, Northern blotting and related molecular assays. |
| Principle / Technology | RNase-suppressing pretreatment followed by phenol/guanidinium lysis, phase separation and alcohol precipitation of RNA. |
| Detection Method | Downstream RNA analysis. |
| Sample Type | Gram-positive bacteria, Gram-negative bacteria, yeast and other non-spore-forming fungi. |
| Performance Range / Specifications | Optimized for bacterial and fungal RNA extraction without lysozyme treatment or ultrasonic disruption; 100 reactions. |
| Specificity | Pretreatment and strong lysis chemistry improve RNA yield and purity from microbial cell walls while protecting RNA integrity. |
| Reaction Conditions / Protocol | Treat the microbial pellet with the pretreatment reagent, add the total RNA extraction reagent, perform phase separation, precipitate, wash and dissolve RNA. |
| Components / Formulation | Microbial RNA pretreatment reagent and phenol/guanidinium total RNA extraction reagent. |
| Package Specifications | 100 reactions. |
| Product Form | Two-component microbial total RNA extraction kit. |
| Quality Control | RNA yield, purity and integrity are evaluated using representative Gram-positive and Gram-negative bacteria. |
| Key Features | Optimized for bacteria and fungi; no lysozyme or sonication required; improved RNA yield and purity; simple workflow. |
| Compatibility | qPCR, RT-PCR, Northern blotting and related RNA-analysis workflows. |
| Application Notes / Precautions | Use RNase-free technique and apply the pretreatment uniformly to the microbial pellet before lysis. |
| Batch-to-Batch Consistency | Microbial RNA yield and integrity are controlled across production batches. |
For research use only, not for clinical use.
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