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| Product Name | Automated 96-Well Plate DNA Extraction Kit |
| Catalog No. | DREK-0020 |
| Description | A high-throughput magnetic bead-based genomic DNA extraction kit pre-configured for automated 96-well plate processing on open-deck liquid handling platforms. The reagent kit includes pre-aliquoted 96-well deep-well plates prefilled with precisely measured reagents, magnetic bead suspensions in optimized dispensing bottles, and plate-compatible elution strips. The chemistry is validated for consistent DNA yield and purity across all 96 wells, enabling parallel processing of large sample cohorts for biobanking, population genomics, and high-throughput genotyping. |
| Intended Use | Automated high-throughput extraction of genomic DNA from whole blood, buffy coat, cultured cells, and tissue homogenates in a 96-well format for large-scale genotyping, GWAS studies, biobanking, and high-throughput sequencing projects. |
| Principle / Technology | Samples are loaded into a 96-well deep-well processing plate containing lysis/binding buffer and proteinase K. The plate is incubated with orbital shaking on the liquid handler deck. Magnetic silica beads are dispensed, and DNA binds under high-salt conditions. The liquid handler performs magnetic separation, aspirates supernatant, and executes a series of ethanol-based wash steps. After final drying, elution buffer is added and purified DNA is collected in a 96-well elution plate. All steps are programmable and require no user intervention after setup. |
| Detection Method | UV spectrophotometry via plate reader; fluorometric quantitation (96-well plate format); agarose gel electrophoresis for representative wells; qPCR for yield uniformity assessment across plate; NGS library quality metrics for validation plates |
| Sample Type | Human whole blood (EDTA or citrate anticoagulated, up to 400 µL per well), buffy coat, cultured mammalian cells, tissue homogenates (pre-prepared); samples must be in single-cell suspension or homogeneous lysate before loading |
| Performance Range / Specifications | Sample throughput: up to 96 samples per run in approximately 90-120 minutes; input: 50-400 µL whole blood; yield: 4-10 µg per well from 200 µL whole blood; A260/A280: 1.75-1.90; well-to-well CV: <10% for DNA yield; plate-to-plate CV: <12%; elution volume: 50-200 µL per well |
| Sensitivity / LOD | Consistent DNA recovery from all 96 wells with outlier rate <5%; detectable DNA from 10 µL blood per well using miniaturized protocol; effective with blood samples stored at 2-8°C for up to 7 days before processing |
| Specificity | Human genomic DNA free of PCR inhibitors; no detectable cross-contamination between adjacent wells; RNase treatment eliminates RNA; heme contamination below detection; consistent fragment size across plate |
| Reaction Conditions / Protocol | Load sample plate with blood samples and lysis/proteinase K master mix; incubate at 56°C with shaking for 20 minutes; add binding buffer and magnetic bead suspension via 8-channel or 96-channel pipetting head; mix; incubate 10 minutes; magnetically separate beads for 3-5 minutes; aspirate supernatant; dispense wash buffer 1; mix; separate; aspirate; dispense wash buffer 2; mix; separate; aspirate; repeat wash buffer 2; air-dry beads on deck for 5-10 minutes; dispense elution buffer; mix; incubate 5 minutes at 56°C; magnetically separate; transfer eluate to PCR plate or storage plate; total deck time: 90-120 minutes |
| Components / Formulation | Lysis/binding buffer (guanidine hydrochloride, Tris-HCl, EDTA, SDS) in bulk bottle, proteinase K solution in bulk bottle, magnetic silica bead suspension in bulk dispensing bottle, wash buffer 1 concentrate in bulk bottle, wash buffer 2 concentrate in bulk bottle, elution buffer (10 mM Tris-HCl pH 8.5, 0.1 mM EDTA) in bulk bottle, 96-well deep-well processing plates (2.2 mL), 96-well elution plates, adhesive plate seals, magnetic separation plate carrier |
| Storage Conditions | Bulk buffers at room temperature (15-25°C); proteinase K at 2-8°C; magnetic bead suspension at 2-8°C; pre-filled plates packaged in sealed foil pouches; unopened pouches stable at room temperature |
| Shelf Life | 18 months for buffers; proteinase K 12 months at 2-8°C after opening; pre-filled plates 12 months at room temperature in sealed pouch; magnetic bead suspension 18 months at 2-8°C |
| Package Specifications | 4 × 96 preparation kits (384 preps total) and 10 × 96 preparation kits (960 preps total); includes pre-filled plates, bulk reagents, and consumables; compatible with major liquid handler brands |
| Product Form | Pre-filled 96-well deep-well plates with aliquoted reagents; bulk liquid reagents for refill; magnetic bead suspension in dispenser bottle; proteinase K in bulk; consumable plates and seals |
| Quality Control | Each lot validated with 96-well test run using pooled human blood: mean yield 6-8 µg/well from 200 µL; A260/A280: 1.75-1.90; well-to-well CV <10%; GAPDH qPCR positive for all 96 wells; no edge effects; no cross-well contamination; endotoxin <0.1 EU/µg DNA |
| Key Features | True walk-away automation with pre-aliquoted reagent plates; validated on major open-deck liquid handlers; consistent well-to-well performance; bulk reagent format for cost-effective operation; magnetic bead chemistry optimized for plate-based magnetic separation; 96-well elution plate format for direct downstream integration |
| Purity | A260/A280: 1.75-1.90; A260/A230: >1.7; well-to-well purity CV <3% |
| Concentration | Adjustable elution volume 50-200 µL; typical concentration 30-80 ng/µL from 200 µL blood input; concentration uniformity across plate verified |
| Activity / Unit Definition | Not applicable for DNA extraction kits |
| Molecular Weight | Not applicable for heterogeneous genomic DNA |
| Source / Origin | Silica-coated magnetic beads from sol-gel synthesis; proteinase K recombinant from Pichia pastoris; all buffer components synthetic, nuclease-free; plates manufactured from virgin polypropylene |
| pH Range / Optimal pH | Lysis buffer pH 6.0-6.5; wash buffers pH 6.5-7.5; elution buffer pH 8.3-8.7 |
| Shipping Conditions | Ambient temperature for pre-filled plates and bulk buffers; proteinase K and magnetic beads shipped with cold packs; standard logistics acceptable |
| Expiration Date / Stability | 18 months unopened; pre-filled plate stability confirmed at 12 months ambient by real-time monitoring; accelerated aging at 40°C supports stability claims; proteinase K activity >85% through expiry period |
| Regulatory / Compliance | For research use only; not for diagnostic procedures; manufactured under ISO 13485 quality management principles; suitable for high-throughput research and biobanking applications |
| Compatibility | Compatible with Tecan Freedom EVO, Hamilton Star, Beckman Biomek, PerkinElmer JANUS, Agilent Bravo, and other open-deck liquid handling platforms; eluted DNA compatible with all downstream genomic applications |
| Recommended Buffer System | Guanidine hydrochloride-based lysis and binding chemistry; ethanol-based wash system; Tris-EDTA elution buffer optimized for plate-based processing |
| Application Notes / Precautions | Optimize liquid handler deck layout before starting; calibrate magnetic separation time for specific magnet type and plate; ensure complete aspiration of wash buffer to avoid ethanol carryover; use plate seals to prevent evaporation during incubations; for viscous samples, reduce input volume or pre-dilute; run a control blood sample in designated well positions for QC monitoring |
| Batch-to-Batch Consistency | Well-to-well CV <10% within lot; lot-to-lot CV <15%; magnetic bead binding capacity controlled per batch; pre-filled plate reagent volumes verified by weight check; proteinase K specific activity verified per batch |
For research use only, not for clinical use.
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