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| Product Name | Anti-Rabbit IgG Magnetic Beads, 2 µm, for Immunoprecipitation |
| Catalog No. | MAGBEA-0025 |
| Description | Superparamagnetic beads (2 µm) conjugated with polyclonal goat anti-rabbit IgG (H+L) for immunoprecipitation of rabbit primary antibodies. Uniform particle size provides consistent performance with binding capacity exceeding 10 µg rabbit IgG per mg beads. Suitable for Co-IP, ChIP, and protein complex analysis with rabbit antibodies. |
| Intended Use | Immunoprecipitation with rabbit antibodies; Co-IP and protein complex analysis; ChIP with rabbit antibodies; target enrichment for mass spectrometry; antibody depletion. |
| Principle / Technology | Goat anti-rabbit IgG (H+L) covalently coupled to superparamagnetic beads; captures rabbit IgG through Fc-region recognition with high specificity |
| Detection Method | 25-50 µL beads per IP; wash 2×; incubate with antibody-lysate 30-60 min RT or overnight 4 °C; magnetic separate; wash 3-5×; elute |
| Components / Formulation | Anti-rabbit IgG magnetic beads (10 mg/mL in PBS, 0.1% BSA, 0.02% NaN₃) |
| Storage Conditions | 2-8 °C; do not freeze; mix thoroughly before use |
| Shelf Life | 24 months |
| Package Specifications | 1 mL, 5 mL, 10 mL suspension |
| Product Form | Brown-black magnetic suspension, 10 mg/mL |
| Key Features | >10 µg rabbit IgG binding per mg beads; 2 µm uniform size; <10 sec magnetic separation; low non-specific binding; goat antibody — no protein A/G interference |
| Purity | Goat anti-rabbit IgG affinity-purified; endotoxin <0.1 EU/mg; no cross-reactivity with mouse, rat, human IgG |
| Concentration | 10 mg/mL suspension |
| Activity / Unit Definition | Binding capacity in µg target per mg beads as specified |
| Molecular Weight | Not applicable — inorganic/organic composite particles |
| Source / Origin | Goat polyclonal anti-rabbit IgG (H+L) |
| pH Range / Optimal pH | pH 6.0–9.0 working range; optimal pH varies by surface chemistry |
| Shipping Conditions | Cold pack 2-8 °C; do not freeze |
| Expiration Date / Stability | 24 months at 2-8 °C; never freeze; do not allow to dry |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 |
| Compatibility | Compatible with rabbit monoclonal and polyclonal antibodies; standard IP lysis buffers |
| Recommended Buffer System | PBS pH 7.4 with 0.02% sodium azide or 20% ethanol for long-term storage |
| Application Notes / Precautions | Vortex thoroughly before each use; avoid drying beads; use appropriate magnetic separation times |
| Batch-to-Batch Consistency | Particle size CV ≤10%; binding capacity within ±15% of reference lot |
For research use only, not for clinical use.
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