Anti-Rabbit IgG Magnetic Beads, 2 µm, for Immunoprecipitation
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Anti-Rabbit IgG Magnetic Beads, 2 µm, for Immunoprecipitation

Cat.No: MAGBEA-0025 Datasheet

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Product Details Related Products
Product Name Anti-Rabbit IgG Magnetic Beads, 2 µm, for Immunoprecipitation
Catalog No. MAGBEA-0025
Description Superparamagnetic beads (2 µm) conjugated with polyclonal goat anti-rabbit IgG (H+L) for immunoprecipitation of rabbit primary antibodies. Uniform particle size provides consistent performance with binding capacity exceeding 10 µg rabbit IgG per mg beads. Suitable for Co-IP, ChIP, and protein complex analysis with rabbit antibodies.
Intended Use Immunoprecipitation with rabbit antibodies; Co-IP and protein complex analysis; ChIP with rabbit antibodies; target enrichment for mass spectrometry; antibody depletion.
Principle / Technology Goat anti-rabbit IgG (H+L) covalently coupled to superparamagnetic beads; captures rabbit IgG through Fc-region recognition with high specificity
Detection Method 25-50 µL beads per IP; wash 2×; incubate with antibody-lysate 30-60 min RT or overnight 4 °C; magnetic separate; wash 3-5×; elute
Components / Formulation Anti-rabbit IgG magnetic beads (10 mg/mL in PBS, 0.1% BSA, 0.02% NaN₃)
Storage Conditions 2-8 °C; do not freeze; mix thoroughly before use
Shelf Life 24 months
Package Specifications 1 mL, 5 mL, 10 mL suspension
Product Form Brown-black magnetic suspension, 10 mg/mL
Key Features >10 µg rabbit IgG binding per mg beads; 2 µm uniform size; <10 sec magnetic separation; low non-specific binding; goat antibody — no protein A/G interference
Purity Goat anti-rabbit IgG affinity-purified; endotoxin <0.1 EU/mg; no cross-reactivity with mouse, rat, human IgG
Concentration 10 mg/mL suspension
Activity / Unit Definition Binding capacity in µg target per mg beads as specified
Molecular Weight Not applicable — inorganic/organic composite particles
Source / Origin Goat polyclonal anti-rabbit IgG (H+L)
pH Range / Optimal pH pH 6.0–9.0 working range; optimal pH varies by surface chemistry
Shipping Conditions Cold pack 2-8 °C; do not freeze
Expiration Date / Stability 24 months at 2-8 °C; never freeze; do not allow to dry
Regulatory / Compliance For laboratory and research use only; RUO; ISO 9001
Compatibility Compatible with rabbit monoclonal and polyclonal antibodies; standard IP lysis buffers
Recommended Buffer System PBS pH 7.4 with 0.02% sodium azide or 20% ethanol for long-term storage
Application Notes / Precautions Vortex thoroughly before each use; avoid drying beads; use appropriate magnetic separation times
Batch-to-Batch Consistency Particle size CV ≤10%; binding capacity within ±15% of reference lot

For research use only, not for clinical use.

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