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| Product Name | α-Amylase (α-AL) Activity Assay Kit (Iodine-starch Colorimetry), 50T/24S |
| Catalog No. | SCAK-YJL-0087 |
| Detection Method | Visible spectrophotometry, Spectrophotometer |
| Storage | Store at 2-8°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
α-Amylase (α-AL) is a key hydrolase enzyme that plays a critical role in carbohydrate metabolism by randomly cleaving α-1,4-glycosidic bonds in starch molecules. This catalytic action breaks down starch into smaller, fermentable carbohydrates such as glucose, maltose, and dextrin, while also reducing the viscosity of starch solutions—earning it the alternative name “liquefying enzyme”.In research settings, accurate measurement of α-Amylase activity is essential for studies spanning multiple fields, including animal physiology (e.g., analyzing digestive enzyme function in serum, plasma, or tissue extracts), plant biology (e.g., investigating starch degradation during seed germination or fruit ripening), and microbial biotechnology (e.g., evaluating enzyme production in fungal or bacterial cultures).The α-Amylase (α-AL) Activity Assay Kit (Iodine-starch Colorimetry) is designed to quantify α-Amylase activity using a well-established visible spectrophotometry method. The principle relies on the specific interaction between iodine and unhydrolyzed starch: iodine binds to residual starch to form a blue-colored complex, and the intensity of this blue color is inversely proportional to the amount of starch hydrolyzed by α-Amylase. By measuring absorbance changes at the appropriate wavelength (typically 570–660 nm), researchers can calculate α-Amylase activity based on the reduction in blue color, ensuring reliable and reproducible results for experimental analysis.Importantly, this kit targets α-Amylase specifically, as β-Amylase (a related starch-hydrolyzing enzyme) can be inactivated by heat treatment (e.g., 70°C for 15 minutes), eliminating potential interference and ensuring the assay measures only α-Amylase activity—a critical feature for precise research data.
detection, ensuring high specificity and consistency in research results.
Compatible with multiple research sample types, including animal serum, plasma, tissue extracts, and plant tissue homogenates (e.g., seeds, leaves), supporting diverse experimental needs across physiology, botany, and biochemistry.
Designed for use with a visible spectrophotometer—an accessible, widely available instrument in most research laboratories—minimizing the need for specialized or high-cost equipment.
Provides a test capacity of 50T/24S (50 tests or 24 samples, depending on experimental setup), offering flexibility for small-scale pilot studies or medium-volume experimental batches.
Stores at 2–8°C with a 6-month shelf life, ensuring long-term usability and reducing the need for frequent reordering, which supports efficient laboratory inventory management.
Includes clear, step-by-step operation guidelines (e.g., sample preparation, reagent dilution, reaction timing) to simplify experimental workflows, even for researchers with limited experience in enzyme activity assays.
High reliability: The iodine-starch colorimetry method has been widely adopted in academic and industrial research, with proven reproducibility (low intra-assay and inter-assay CV) to ensure consistent data across experiments.
Cost-effective: The 50T/24S format balances test volume and price, making it suitable for laboratories with moderate sample throughput without compromising on result quality.
Time-efficient: The assay protocol involves straightforward steps (e.g., pre-warming substrates, short incubation times) and can be completed within a reasonable timeframe (typically 40–60 minutes total), reducing experimental turnaround.
Minimal interference: The option to inactivate β-Amylase via heat treatment eliminates cross-reactivity, ensuring the measured activity is specific to α-Amylase—critical for avoiding misleading data in mixed-enzyme samples.
User-friendly: No specialized technical skills are required to operate the kit, and all necessary reagents are optimized for compatibility, reducing the risk of human error during sample handling or reagent preparation.
Research-focused design: Tailored for research use only (not for clinical applications), the kit’s specifications align with standard laboratory research needs, such as supporting sample dilution (e.g., for high-activity samples like tissue homogenates) to ensure absorbance values fall within the detectable range.
For research use only, not for clinical use.
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