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| Product Name | All-in-One Red Tracking RT SuperMix for qPCR with gDNA Removal |
| Catalog No. | NATR-HMM-0171 |
| Description | An all-in-one qPCR reverse-transcription premix with a red tracking dye, combining genomic DNA removal and cDNA synthesis in a single tube. |
| Intended Use | First-strand cDNA synthesis; reverse transcription; gene expression analysis; two-step RT-qPCR. |
| Principle / Technology | Genomic DNA removal and RNA reverse transcription occur in one premixed tube, while a red tracking dye provides visual confirmation during pipetting. |
| Sample Type | RNA from animal, plant or bacterial samples. |
| Performance Range / Specifications | 100 reactions; one-tube workflow completed in approximately 15 minutes. |
| Specificity | The red tracking dye does not interfere with dye-based or probe-based qPCR detection. |
| Reaction Conditions / Protocol | Add RNA template and nuclease-free water to the all-in-one supermix and complete genomic DNA removal and reverse transcription in approximately 15 minutes. |
| Components / Formulation | All-in-one red RT supermix containing genomic DNA removal activity, reverse-transcription components and a visible tracking dye. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions. |
| Product Form | Red tracking all-in-one RT supermix. |
| Quality Control | Performance is assessed by reverse transcription followed by dye-based and probe-based qPCR. |
| Key Features | Visible pipetting; one-tube operation; approximately 15-minute workflow; broad RNA-source compatibility; dye- and probe-qPCR compatibility. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with dye-based and probe-based qPCR assays. |
| Application Notes / Precautions | Optimized for two-step RT-qPCR rather than long-fragment PCR. Random-primer activity may generate short nonspecific cDNA products in long-target applications. |
For research use only, not for clinical use.
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