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| Product Name | Advanced Lipid Transfection Reagent, for Difficult-to-Transfect Cells |
| Catalog No. | ATR-CTR-0002 |
| Description | A third-generation cationic lipid nanoparticle delivery system with enhanced efficiency for hard-to-transfect cell types, primary cells, and co-transfection of multiple plasmids simultaneously. |
| Intended Use | Transfection of primary cells, stem cells, and recalcitrant cell lines where traditional lipid reagents yield low efficiency; simultaneous delivery of multiple plasmids for CRISPR genome editing, virus production, and multi-gene expression systems. |
| Principle / Technology | Proprietary lipid formulation enhances endosomal escape efficiency and reduces cytotoxicity compared to earlier generation lipid reagents; optimized lipid-to-DNA ratio and complex formation conditions improve delivery to the nucleus. |
| Detection Method | Multi-color fluorescence reporter assay for co-transfection efficiency, CRISPR editing efficiency by T7E1 assay or sequencing |
| Sample Type | Primary fibroblasts, HUVEC, MEFs, iPSCs, Jurkat, K562, THP-1, and other difficult-to-transfect cell lines |
| Performance Range / Specifications | Transfection efficiency 30–60% for primary cells (versus <10% with standard reagents); >80% for HEK293; co-transfection of 3 plasmids with >50% cells expressing all 3 |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Dilute DNA in Opti-MEM; dilute reagent separately (0.75–1.5 µL per µg DNA); combine and incubate 15 min; add to cells; change medium after 4–6 hours for sensitive cells. |
| Components / Formulation | Proprietary lipid nanoparticle formulation, ethanol-water vehicle, 0.2 µm filtered |
| Storage Conditions | 2–8°C; do not freeze; warm to room temperature and vortex before use |
| Shelf Life | 12 months |
| Package Specifications | 0.75 mL, 1.5 mL, 5 mL |
| Product Form | Liquid, sterile-filtered |
| Quality Control | Transfection efficiency validation with HEK293, HeLa, HUVEC, and THP-1 reference cells; cytotoxicity testing; sterility; endotoxin |
| Key Features | Enhanced efficiency for difficult cells; supports co-transfection; low cytotoxicity; validated for CRISPR delivery; compatible with serum-containing media |
For research use only, not for clinical use.
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