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| Product Name | 8-Minute Plasmid DNA Mini Extraction Kit |
| Catalog No. | DREK-0086 |
| Description | A rapid spin-column kit for purifying plasmid DNA from overnight bacterial cultures. Optimized alkaline lysis, color-indicated neutralization and silica-membrane binding support fast recovery of clean plasmid DNA. |
| Intended Use | Plasmid DNA purification for restriction digestion, PCR, sequencing, ligation, transformation and routine cell transfection research. |
| Principle / Technology | Alkaline lysis followed by color-indicated neutralization and selective silica-membrane binding. |
| Detection Method | Downstream DNA analysis. |
| Sample Type | 1-4 mL overnight bacterial culture. |
| Performance Range / Specifications | Approximately 8-minute workflow; plasmid yield up to 25 µg; 150 reactions. |
| Specificity | Silica membrane selectively binds plasmid DNA while wash steps remove proteins, polysaccharides and other contaminants. |
| Reaction Conditions / Protocol | Harvest bacteria, resuspend, lyse and neutralize with color indication, clarify the lysate, bind plasmid DNA to the column, wash and elute. |
| Components / Formulation | Resuspension, lysis, neutralization, wash and elution buffers; silica spin columns and collection tubes. |
| Storage Conditions | Store at 15-25°C. |
| Package Specifications | 150 reactions. |
| Product Form | Spin-column plasmid DNA extraction kit. |
| Quality Control | Yield, purity and downstream functional performance are verified using representative plasmids. |
| Key Features | Approximately 8-minute extraction; yield up to 25 µg; visual lysis/neutralization indicator; compact precipitate; high-purity DNA. |
| Shipping Conditions | Ship at room temperature. |
| Compatibility | Restriction digestion, PCR, sequencing, ligation, transformation and routine transfection workflows. |
| Application Notes / Precautions | Use an appropriate culture volume for plasmid copy number and avoid overloading the binding column. |
| Batch-to-Batch Consistency | Yield and purity are controlled across production batches. |
For research use only, not for clinical use.
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