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| Product Name | 6-Minute One-Step Plasmid DNA Mini Extraction Kit |
| Catalog No. | DREK-0090 |
| Description | A rapid plasmid mini-prep kit that combines bacterial resuspension, lysis and neutralization into a single preparation step before silica-column purification. |
| Intended Use | Plasmid DNA purification for restriction digestion, PCR, sequencing, ligation and transformation. |
| Principle / Technology | One-step alkaline lysis followed by selective silica-membrane binding and washing. |
| Detection Method | Downstream DNA analysis. |
| Sample Type | 1-5 mL overnight endA-negative Escherichia coli culture. |
| Performance Range / Specifications | Approximately 6-minute workflow; typical yield 10-35 µg; maximum column binding capacity 35 µg; recommended plasmid size up to 10 kb; 200 reactions. |
| Specificity | Silica-membrane binding and wash chemistry remove proteins and genomic DNA while enriching plasmid DNA. |
| Reaction Conditions / Protocol | Harvest bacteria, add the chilled one-step lysis buffer and vortex immediately, load the clarified material onto the column, wash and elute. |
| Components / Formulation | One-step lysis reagent, wash and elution buffers, silica spin columns and collection tubes. |
| Storage Conditions | Store the kit at 15-25°C. After the active mix is added to the one-step lysis buffer, store it at 2-8°C and use within 4 months; the active mix is recommended at 2-8°C or −20°C for longer storage. |
| Package Specifications | 200 reactions. |
| Product Form | Rapid plasmid DNA spin-column kit. |
| Quality Control | Yield, DNA integrity and downstream functional performance are verified using representative plasmids. |
| Key Features | One-step lysis; approximately 6-minute extraction; yield up to 35 µg; simplified workflow; high-quality plasmid DNA. |
| Compatibility | Restriction digestion, PCR, sequencing, ligation and transformation workflows. |
| Application Notes / Precautions | Designed for endA-negative E. coli. Keep the prepared lysis buffer cold and vortex immediately after addition to minimize genomic DNA carryover. |
| Batch-to-Batch Consistency | Yield and purity are controlled across production batches. |
For research use only, not for clinical use.
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