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| Product Name | 5-Minute Universal DNA Ligation Mix |
| Catalog No. | NATR-HMM-0167 |
| Description | A ready-to-use 2× DNA ligation premix containing T4 DNA ligase, optimized reaction buffer and ligation enhancer for rapid assembly of diverse DNA ends. |
| Intended Use | Sticky-end ligation, blunt-end ligation, TA ligation and linker or adapter ligation. |
| Principle / Technology | T4 DNA ligase catalyzes phosphodiester-bond formation between adjacent 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA. |
| Sample Type | DNA fragments with cohesive, blunt or single-base overhanging ends; linkers and adapters. |
| Reaction Conditions / Protocol | Incubate at 25°C for 5 minutes. The ligation product can be used directly to transform compatible chemically competent cells. |
| Components / Formulation | 2× universal ligation mix containing T4 DNA ligase, optimized buffer and ligation enhancer. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 50 reactions: 250 μL; 100 reactions: 2 × 250 μL. |
| Product Form | 2× ready-to-use ligation premix. |
| Key Features | Five-minute ligation; broad end compatibility; ready-to-use formulation; high cloning efficiency; direct transformation compatibility. |
| Compatibility | Compatible with sticky, blunt and TA ligation, as well as linker and adapter joining. |
For research use only, not for clinical use.
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